Carbamoyl-phosphate synthetase gene of coryneform bacteria and method for producing L-arginine

Abstract

A DNA fragment which encodes a polypeptide defined in the following (a) or (b), and a polypeptide defined in the following (c) or (d): (a) a polypeptide which has at least the amino acid sequence of the amino acid numbers 50 to 393 in SEQ ID NO: 2 shown in Sequence Listing, (b) a polypeptide which has at least the amino acid sequence of the amino acid numbers 50 to 393 in SEQ ID No: 2 shown in Sequence Listing including substitution, deletion, insertion, addition, or inversion of one or several amino acids, and can constitute a protein having a carbamoyl-phosphate synthetase activity with a large subunit of carbamoyl-phosphate synthetase having the amino acid sequence comprising at least the amino acid numbers 55 to 1113 of SEQ ID NO: 3, (c) a polypeptide which has the amino acid sequence comprising at least the amino acid numbers 55 to 1113 of SEQ ID NO: 3 shown in Sequence Listing, (d) a polypeptide which has the amino acid sequence comprising at least the amino acid numbers 55 to 1113 of SEQ ID NO: 3 shown in Sequence Listing including substitution, deletion, insertion, addition, or inversion of one or several amino acids, and can constitute a protein having a carbamoyl-phosphate synthetase activity with a small subunit of carbamoyl-phosphate synthetase having the amino acid sequence of the amino acid numbers 50 to 393 in SEQ ID NO: 2.

What is claimed is:  
     
       1. A method for producing L-arginine, comprising: 
       culturing a host cell comprising the nucleic acid sequence of SEQ ID NO: 1 or a fragment thereof that encodes a polypeptide with carbamoyl-phosphate synthetase activity in a medium and under conditions suitable for production of L-arginine, and  
       collecting the L-arginine.  
     
     
       2. A method for producing L-arginine, comprising: 
       culturing, in a medium and under conditions suitable for production of L-arginine, a host cell comprising a variant or mutant of the nucleic acid sequence of SEQ ID NO: 1 that encodes a polypeptide with carbamoyl-phosphate synthetase activity, and that hybridizes under stringent conditions of 60° C., 1×SSC, and 0.1% SDS with the nucleic acid sequence of SEQ ID NO: 1; and  
       collecting the L-arginine.  
     
     
       3. The method of claim  1 , wherein said host cell is a coryneform bacterium. 
     
     
       4. The method of claim  2 , wherein said host cell is a coryneform bacterium.