Polypeptides having kinase activity, their preparation and use

Abstract

This invention relates to new polypeptides which exhibit kinase activity or, more specifically, which show phosphoinositide (PI) 3-kinase activity. Such polypeptides are involved in pathways responsible for cellular growth and differentiation. An isolated polypeptide which possesses PI3-kinase activity when produced by recombinant production in insect cells is disclosed.

Claims  
     
       1. A method for making an antibody which specifically binds to a polypeptide which has PI3 kinase activity and a molecular weight of about 110 kilodaltons as determined by SDS-PAGE, comprising administering an immunogen to a subject animal, wherein said immunogen comprises a peptide, the amino acid sequence of which consists of the amino acid sequence set forth at SEQ ID NO: 32, in an amount sufficient to provoke an antibody response against said immunogen by said subject animal, and isolating said antibody from said subject animal. 
     
     
       2. The method of claim  1 , wherein said immunogen comprises keyhole limpet hemocyanin complexed to said peptide. 
     
     
       3. An isolated antibody which specifically binds to the PI3 kinase having the amino acid sequence set forth in SEQ ID. NO: 32, wherein said antibody binds to said PI 3  kinase at an epitope positioned in the region defined by amino acids 1-128 of SEQ ID. NO: 32. 
     
     
       4. The antibody of claim  3 , wherein said epitope is positioned in the region defined by amino acids 19-110 of SEQ. ID. NO: 32. 
     
     
       5. The antibody of claim  3 , wherein said epitope is positioned in the region defined by amino acids 19-108 of SEQ. ID. NO: 32. 
     
     
       6. The antibody of claim  3 , wherein said epitope is positioned in the region defined by amino acids 1-108 of SEQ ID. NO: 32. 
     
     
       7. An antibody which binds to a polypeptide which has PI3 kinase activity and a molecular weight of about 110 kilodaltons as determined by SDS-PAGE, produced by immunizing a subject animal with an immunogen, said immunogen comprising a peptide, the amino acid sequence of which consists of the amino acid sequence set forth at SEQ ID NO: 32. 
     
     
       8. The antibody of claim  7 , wherein said immunogen comprises keyhold limpet hemocyanin complexed to said peptide. 
     
     
       9. The antibody of claim  7 , wherein said antibody is a monoclonal antibody. 
     
     
       10. A method for determining if a polypeptide which has PI3 kinase activity and a molecular weight of about 110 kilodaltons as determined by SDS-PAGE is present in a sample, comprising contacting said sample with the antibody of claim  7  and determining binding of said antibody as a determination of said polypeptide in said sample.