US6309650B1ExpiredUtility

Attenuated Japanese encephalitis virus adapted to Vero cell and a Japanese encephalitis vaccine

79
Assignee: CHEIL JEDANG CORPPriority: Aug 28, 1997Filed: Aug 25, 1998Granted: Oct 30, 2001
Est. expiryAug 28, 2017(expired)· nominal 20-yr term from priority
Inventors:Hyun-Su KimWang-Don YooSoo-Ok KimSung-Hee LeeSang Bum MoonSun-Pyo HongYong-Cheol ShinYong Ju ChungKenneth H. EckelsBruce InnisJoseph R. PuniakLeonard N. BinnAshok K. SrivastavaDoria R. Dubois
C12N 7/00C07K 14/005A61K 39/12Y02A50/30C12N 2770/24134C12N 2770/24151A61K 2039/5254A61K 2039/55505C12N 2770/24122
79
PatentIndex Score
56
Cited by
5
References
12
Claims

Abstract

An attenuated Japanese encephalitis virus adapted to Vero cell by passages on Vero cell is disclosed. A Japanese encephalitis vaccine comprising said attenuated virus is also disclosed.

Claims

exact text as granted — not AI-modified
What is claimed is:  
     
       1. An attenuated Japanese encephalitis virus adapted to Vero cell by passages on Vero cell wherein said virus has a multiplicity of more than 1×10(7) PFU/ml in Vero cells and LD 50 /pfu for young adult mouse is less than 0.000001. 
     
     
       2. A Japanese encephalitis vaccine comprising the attenuated Japanese encephalitis virus according to claim  1 . 
     
     
       3. The vaccine according to claim  2 , which further comprises pharmaceutically acceptable additives. 
     
     
       4. The vaccine according to claim  2  wherein the virus is inactivated by an inactivating. agent. 
     
     
       5. The vaccine according to claim  4 , which further comprises pharmaceutically acceptable additives. 
     
     
       6. The vaccine according to claim  2  wherein the virus is live-attenuated JE virus untreated by an inactivating agent. 
     
     
       7. The vaccine according to claim  6 , which further comprises pharmaceutically acceptable additives. 
     
     
       8. An attenuated Japanese encephalitis virus adapted to Vero cell by passages on Vero cell which is CJ50003. 
     
     
       9. A Japanese encephalitis vaccine comprising the attenuated Japanese encephalitis virus according to claim  8 . 
     
     
       10. The vaccine according to claim  8 , wherein the virus is inactivated by an inactivating agent. 
     
     
       11. The vaccine according to claim  8 , wherein the virus is live-attenuated JE virus untreated by an inactivating agent. 
     
     
       12. The vaccine according to claim  9 , which further comprises pharmaceutically acceptable additives.

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