DNA encoding—candida albicans integrin-like protein

Abstract

An isolated and purified DNA molecule encoding Candida albicans protein with integrin-like motifs, the protein itself, antibodies thereto, and methods of use, are provided.

What is claimed is:  
     
       1. An isolated and purified DNA molecule encoding a  Candida albicans  protein with integrin-like motifs that hybridizes to DNA complementary to DNA having SEQ ID NO:1 under the stringency conditions of hybridization in buffer containing 5×SSC, 5×Denhardt's, 0.5% SDS, 1 mg salmon sperm/25 mls of hybridization solution incubated at 65 ° C. overnight, followed by high stringency washing with 0.2×SSC/0.1% SDS at 65° C., wherein said protein binds epithelial cells. 
     
     
       2. The DNA molecule of  claim 1  wherein the  Candida albicans  protein with integrin-like motifs contains an I domain, two EF-hand divalent cation binding sites, a sequence sufficient to encode a transmembrane domain, an internal RGD tripeptide, and a carboxy-terminal sequence having a single tyrosine residue. 
     
     
       3. An isolated and purified DNA molecule encoding the  Candida albicans  protein with integrin-like motifs wherein the protein has an amino acid sequence represented by SEQ ID NO:2. 
     
     
       4. An isolated and purified DNA molecule having SEQ ID NO:1. 
     
     
       5. A vector comprising the DNA of  claim 4 . 
     
     
       6. A cell line transformed by an extrachromosomal plasmid containing non-native DNA encoding a  Candida albicans  protein with integrin-like motifs, wherein said DNA hybridizes with DNA complementary to DNA having SEQ ID NO:1 under the stringency conditions of hybridization in buffer containing 5×SSC, 5×Denhardt's, 0.5% SDS, 1 mg salmon sperm/25 mls of hybridization solution incubated at 65° C. overnight, followed by high stringency washing with 0.2×SSC/0.1% SDS at 65° C., wherein said protein binds epithelial cells. 
     
     
       7. The cell line of  claim 6  wherein the  Candida albicans  protein with integrin-like motifs contains an I domain, two EF-hand divalent cation binding sites, a sequence sufficient to encode a transmembrane domain, an internal RGD tripeptide, and a carboxy-terminal sequence having a single tyrosine residue. 
     
     
       8. The cell line of  claim 6  which is  S. cerevisiae.    
     
     
       9. A cell line transformed by an extrachromosomal plasmid containing non-native DNA encoding the  Candida albicans  protein with integrin-like motifs, wherein the protein has the amino acid sequence represented by SEQ ID NO:2. 
     
     
       10. The cell line of  claim 9  which is  S. cerevisiae.    
     
     
       11. A cell line transformed by an extrachromosomal plasmid containing non-native DNA encoding the  Candida albicans  protein with integrin-like motifs, the DNA having SEQ ID NO:1. 
     
     
       12. The cell line of  claim 11  which is  S. cerevisiae.