US6355781B1ExpiredUtility

Mammalian growth factor

27
Assignee: UNIV NEW YORKPriority: Jun 16, 1987Filed: Dec 31, 1996Granted: Mar 12, 2002
Est. expiryJun 16, 2007(expired)· nominal 20-yr term from priority
Inventors:Claudio BasilicoPasquale Delli Bovi
C07K 14/50
27
PatentIndex Score
0
Cited by
1
References
1
Claims

Abstract

A homogeneous K-FGF polypeptide having a molecular weight of about 19,000 daltons when analyzed by SDS polyacrylamide gel electrophoresis, wherein the polypeptide is substantially free of oligosaccharide moieties attached to the polypeptide, appears as a single band on SDS-PAGE, exhibits a detectable level of mitogenic activity on growth arrested cells in a 3H-thymidine uptake assay, and is substantially free from other mammalian proteins, is provided.

Claims

exact text as granted — not AI-modified
What is claimed is:  
     
       1. A homogeneous K-FGF polypeptide having a molecular weight of about 19,000 daltons when produced by culturing COS-1 cells containing the plasmid p9BKS3A under the conditions suitable for expression of the protein and in the presence of tunicamycin.

Cited by (0)

No later patents cite this yet.

References (0)

No backward citations on record.