Nucleotide sequences of HIV-1 type (or subtype) O retrovirus antigens

Abstract

An HIV-1 type (or subtype) O retrovirus protein, or a natural or synthetic polypeptide or peptide including at least a part of said protein, which is capable of being recognised by antibodies isolated from a serum resulting from infection by an HIV-1 type O VAU strain or an HIV-1 type (or subtype) O DUR strain.

What is claimed is:  
     
       1. A purified HIV-1 DUR isolate, which is the isolate deposited at C.N.C.M. under the accession no. I-1542. 
     
     
       2. A purified nucleic acid comprising the sequence of SEQ ID NO:63. 
     
     
       3. A purified nucleic acid comprising the sequence of SEQ ID NO:64. 
     
     
       4. A purified HIV-1 Env peptide comprising a sequence selected from the group consisting of: 
       CysValArgProGlyAsnAsnSerValLysGluIleLysIleGlyProMetAlaTrpTyrSerMetGlnIleGluArgGluGlyLysGlyAlaAsnSerArgThrAlaPheCys (SEQ ID NO:93);  
       CysLysAsnArgLeuIleCys (SEQ ID NO:5);  
       ArgLeuLeuAlaLeuGluThrPheIleGInAsnTrpTrpLeuLeuAsnLeuTrpGlyCysLysAsnArgLeuIleCys (SEQ ID NO:6);  
       ArgAlaArgLeuLeuAlaLeuGluThrPheIleGlnAsnGlnGlnLeuLeuAsnLeuTrpGlyCysLysAsnArgLeuIleCysTyrThrSerValLysTrpAsnLysThr (SEQ ID NO:7);  
       LysGluIleLysIle (SEQ ID NO:23);  
       GluArgGluGlyLysGlyAlaAsn (SEQ ID NO:24);  
       CysValArgProGlyAsnAsnSerValLysGluIleLysIle (SEQ ID NO:25);  
       GlnIleGluArgGluGlyLysGlyAlaAsnSerArg (SEQ ID NO:26);  
       ArgLeuLeuAlaLeuGluThrLeuMetGlnAsnGlnGlnLeu (SEQ ID NO:29);  
       LeuAsnLeuTrpGlyCysArgGlyLysAlaIleCysTyrThrSerValGlnTrpAsnGluThrTrpGly (SEQ ID NO:30);  
       CysArgGlyLysAlaIle (SEQ ID NO:31);  
       SerValGlnTrpAsn (SEQ ID NO:32);  
       ArgLeuLeuAlaLeuGluThrLeuMetGlnAsnGlnGlnLeuLeuAsnLeuTrpGlyCysArgGlyLysAlaIleCysTyrThrSer (SEQ ID NO:33);  
       GlnAsnGlnGlnLeuLeuAsnLeuTrpGlyCysArgGlyLysAlaIleCysTyrThrSerValGlnTrpAsn (SEQ ID NO:34);  
       ThrPheIleGlnAsn (SEQ ID NO:40); and  
       TrpGlyCysLysAsnArg (SEQ ID NO:41).  
     
     
       5. An oligonucleotide primer selected from the group consisting of: 
       ATTCCAATACACTATTGTGCTCCA (SEQ ID NO:42);  
       AAAGAATTCTCCATGACTGTTAAA (SEQ ID NO:43);  
       GGTATAGTGCAACAGCAGGACAAC (SEQ ID NO:44);  
       AGAGGCCCATTCATCTAACTC (SEQ ID NO:45); and sequences complementary to these sequences.  
     
     
       6. A probe for HIV group O, wherein said probe is at least 90% identical to SEQ ID NO:63. 
     
     
       7. A purified HIV-1 isolate comprising Env protein, wherein said Env protein comprises a sequence selected from the group consisting of: 
       CysValArgProGlyAsnAsnSerValLysGluIleLysIleGlyProMetAlaTrpTyrSerMetGlnIleGluArgGluGlyLysGlyAlaAsnSerArgThrAlaPheCys (SEQ ID NO:93);  
       CysLysAsnArgLeuIleCys (SEQ ID NO:5);  
       ArgLeuLeuAlaLeuGluThrPheIleGlnAsnTrpTrpLeuLeuAsnLeuTrpGlyCysLysAsnArgLeuIleCys (SEQ ID NO:6);  
       ArgAlaArgLeuLeuAlaLeuGluThrPheIleGlnAsnGlnGlnLeuLeuAsnLeuTrpGlyCysLysAsnArgLeuIleCysTyrThrSerValLysTrpAsnLysThr (SEQ ID NO:7);  
       LysGluIleLysIle (SEQ ID NO:23);  
       GluArgGluGlyLysGlyAlaAsn (SEQ ID NO:24);  
       CysValArgProGlyAsnAsnSerValLysGluIleLysIle (SEQ ID NO:25);  
       GlnIleGluArgGluGlyLysGlyAlaAsnSerArg (SEQ ID NO:26);  
       ArgLeuLeuAlaLeuGluThrLeuMetGlnAsnGlnGlnLeu (SEQ ID NO:29);  
       LeuAsnLeuTrpGlyCysArgGlyLysAlaIleCysTyrThrSerValGlnTrpAsnGluThrTrpGly (SEQ ID NO:30);  
       CysArgGlyLysAlaIle (SEQ ID NO:31);  
       SerValGlnTrpAsn (SEQ ID NO:32);  
       ArgLeuLeuAlaLeuGluThrLeuMetGlnAsnGlnGlnLeuLeuAsnLeuTrpGlyCysArgGlyLysAlaIleCysTyrThrSer (SEQ ID NO:33);  
       GlnAsnGlnGlnLeuLeuAsnLeuTrpGlyCysArgGlyLysAlaIleCysTyrThrSerValGlnTrpAsn (SEQ ID NO:34);  
       ThrPheIleGlnAsn (SEQ ID NO:40); and  
       TrpGlyCysLysAsnArg (SEQ ID NO:41).  
     
     
       8. A probe for HIV group O, wherein said probe encodes an Env peptide comprising a sequence selected from the group consisting of: 
       CysValArgProGlyAsnAsnSerValLysGluIleLysIleGlyProMetAlaTrpTyrSerMetGlnIleGluArgGluGlyLysGlyAlaAsnSerArgThrAlaPheCys (SEQ ID NO:93);  
       CysLysAsnArgLeuIleCys (SEQ ID NO:5);  
       ArgLeuLeuAlaLeuGluThrPheIleGlnAsnTrpTrpLeuLeuAsnLeuTrpGlyCysLysAsnArgLeuIleCys (SEQ ID NO:6);  
       ArgAlaArgLeuLeuAlaLeuGluThrPheIleGlnAsnGlnGlnLeuLeuAsnLeuTrpGlyCysLysAsnArgLeuIleCysTyrThrSerValLysTrpAsnLysThr (SEQ ID NO:7);  
       LysGluIleLysIle (SEQ ID NO:23);  
       GluArgGluGlyLysGlyAlaAsn (SEQ ID NO:24);  
       CysValArgProGlyAsnAsnSerValLysGluIleLysIle (SEQ ID NO:25);  
       GlnIleGluArgGluGlyLysGlyAlaAsnSerArg (SEQ ID NO:26);  
       ArgLeuLeuAlaLeuGluThrLeuMetGlnAsnGlnGlnLeu (SEQ ID NO:29);  
       LeuAsnLeuTrpGlyCysArgGlyLysAlaIleCysTyrThrSerValGlnTrpAsnGluThrTrpGly (SEQ ID NO:30);  
       CysArgGlyLysAlaIle (SEQ ID NO:31);  
       SerValGlnTrpAsn (SEQ ID NO:32);  
       ArgLeuLeuAlaLeuGluThrLeuMetGlnAsnGlnGlnLeuLeuAsnLeuTrpGlyCysArgGlyLysAlaIleCysTyrThrSer (SEQ ID NO:33);  
       GlnAsnGlnGlnLeuLeuAsnLeuTrpGlyCysArgGlyLysAlaIleCysTyrThrSerValGlnTrpAsn (SEQ ID NO:34);  
       ThrPheIleGlnAsn (SEQ ID NO:40); and  
       TrpGlyCysLysAsnArg (SEQ ID NO:41).  
     
     
       9. A purified HIV-1 env gene, wherein said gene encodes a polypeptide comprising a sequence selected from the group consisting of: 
       CysValArgProGlyAsnAsnSerValLysGluIleLysIleGlyProMetAlaTrpTyrSerMetGlnIleGluArgGluGlyLysGlyAlaAsnSerArgThrAlaPheCys (SEQ ID NO:93);  
       CysLysAsnArgLeuIleCys (SEQ ID NO:5);  
       ArgLeuLeuAlaLeuGluThrPheIleGlnAsnTrpTrpLeuLeuAsnLeuTrpGlyCysLysAsnArgLeuIleCys (SEQ ID NO:6);  
       ArgAlaArgLeuLeuAlaLeuGluThrPheIleGlnAsnGlnGlnLeuLeuAsnLeuTrpGlyCysLysAsnArgLeuIleCysTyrThrSerValLysTrpAsnLysThr (SEQ ID NO:7);  
       LysGluIleLysIle (SEQ ID NO:23);  
       GluArgGluGlyLysGlyAlaAsn (SEQ ID NO:24);  
       CysValArgProGlyAsnAsnSerValLysGluIleLysIle (SEQ ID NO:25);  
       GlnIleGluArgGluGlyLysGlyAlaAsnSerArg (SEQ ID NO:26);  
       ArgLeuLeuAlaLeuGluThrLeuMetGlnAsnGlnGlnLeu (SEQ ID NO:29);  
       LeuAsnLeuTrpGlyCysArgGlyLysAlaIleCysTyrThrSerValGlnTrpAsnGluThrTrpGly (SEQ ID NO:30);  
       CysArgGlyLysAlaIle (SEQ ID NO:31);  
       SerValGlnTrpAsn (SEQ ID NO:32);  
       ArgLeuLeuAlaLeuGluThrLeuMetGlnAsnGlnGlnLeuLeuAsnLeuTrpGlyCysArgGlyLysAlaIleCysTyrThrSer (SEQ ID NO:33);  
       GlnAsnGlnGlnLeuLeuAsnLeuTrpGlyCysArgGlyLysAlaIleCysTyrThrSerValGlnTrpAsn (SEQ ID NO:34);  
       ThrPheIleGlnAsn (SEQ ID NO:40); and  
       TrpGlyCysLysAsnArg (SEQ ID NO:41).  
     
     
       10. A purified HIV-1 nucleic acid, wherein said nucleic acid encodes a polypeptide comprising a sequence selected from the group consisting of: 
       CysValArgProGlyAsnAsnSerValLysGluIleLysIleGlyProMetAlaTrpTyrSerMetGlnIleGluArgGluGlyLysGlyAlaAsnSerArgThrAlaPheCys (SEQ ID NO:93);  
       CysLysAsnArgLeuIleCys (SEQ ID NO:5);  
       ArgLeuLeuAlaLeuGluThrPheIleGlnAsnTrpTrpLeuLeuAsnLeuTrpGlyCysLysAsnArgLeuIleCys (SEQ ID NO:6);  
       ArgAlaArgLeuLeuAlaLeuGluThrPheIleGlnAsnGlnGlnLeuLeuAsnLeuTrpGlyCysLysAsnArgLeuIleCysTyrThrSerValLysTrpAsnLysThr (SEQ ID NO:7);  
       LysGluIleLysIle (SEQ ID NO:23);  
       GluArgGluGlyLysGlyAlaAsn (SEQ ID NO:24);  
       CysValArgProGlyAsnAsnSerValLysGluIleLysIle (SEQ ID NO:25);  
       GlnIleGluArgGluGlyLysGlyAlaAsnSerArg (SEQ ID NO:26);  
       ArgLeuLeuAlaLeuGluThrLeuMetGlnAsnGlnGlnLeu (SEQ ID NO:29);  
       LeuAsnLeuTrpGlyCysArgGlyLysAlaIleCysTyrThrSerValGlnTrpAsnGluThrTrpGly (SEQ ID NO:30);  
       CysArgGlyLysAlaIle (SEQ ID NO:31);  
       SerValGlnTrpAsn (SEQ ID NO:32);  
       ArgLeuLeuAlaLeuGluThrLeuMetGlnAsnGlnGlnLeuLeuAsnLeuTrpGlyCysArgGlyLysAlaIleCysTyrThrSer (SEQ ID NO:33);  
       GlnAsnGlnGlnLeuLeuAsnLeuTrpGlyCysArgGlyLysAlaIleCysTyrThrSerValGlnTrpAsn (SEQ ID NO:34);  
       ThrPheIleGlnAsn (SEQ ID NO:40); and  
       TrpGlyCysLysAsnArg (SEQ ID NO:41).  
     
     
       11. A method for the in vitro diagnosis of an infection caused by an HIV-1 (VAU)  virus, said process comprising: 
       (A) obtaining a biological sample comprising antibodies;  
       (B) contacting said sample with at least one peptide of  claim 4 ; and  
       (C) detecting the immunological complexes formed.