Coated polymer article and its use

Abstract

A polymer article having structure (I), where P comprises the basic polymer and groups -L-CH(-X(R1)p)-CH2(-Y(R2)p); L is a part of a pending group utilized for introducing -X(R1)p and -Y(R2)p; X and Y are halogen, N, S and O; R1 is hydrogen, alkyl, acyl or R2 when X is N, O or S; R2 is hydrogen, alkyl, alkylaryl or arylalkyl in which the alkyl part may contain 1-18 carbons, or -R3(-NH-CR4=O)q or -R3(-NH2)q, -CR4=O or poly alkyloxy that may have been terminally acylated or alkylated; p is an integer 0-3, with the provisos a) p depends on X being halogen, O, S or N, and b) that if several groups R2 are present they may be identical or different; R3 is alkyl, -O-alkyl, hydroxyalkyl, phenylalkyl, with up to 18 carbon atoms in the alkyl part, and R4 is C1-18 alkyl. q is an integer>O representing that one or more hydrogens in R3 may have been replaced with -NH2 or -NH-CR4=O. The polymer article may be used as a chromatography separation medium or in solide phase oligonucleotide synthesis.

What is claimed is:  
     
       1. A coated polymer article wherein the coating is covalently bound to the polymer surface via double bonds of the polymer, comprising                    
       a) P comprises a basic polymer and further groups  
       
         
           —L—CH(—X(R 1 ) p )—CH 2 (—Y(R 2 ) p );  
         
       
       b) L is a part of a pending group i) projecting from the basic polymer and ii) comprising a double bond that has been utilized for introducing —X(R 1 ) p  and —Y(R 2 ) p ;  
       c) X and Y are independently selected from halogens, in particular Br, and N, S and O;  
       d) R 1  is hydrogen, a straight, branched or cyclic alkyl or acyl group, such as C 1 -C 10  alkyl, C 1 -C 18  acyl, or R 2  when X is N, O or S with particular emphasis for X equals Y equals O;  
       e) R 2  is hydrogen, straight, branched or cyclic alkyl such as C 1 -C 18  alkyl, —R 3 (—NH 2 ) q , alkylaryl or arylalkyl in which the alkyl part may contain 1-18 carbon atoms, —R 3 (—NH—CR 4 ═O) q , —CR 4 ═O or poly lower alkyloxy groups that may have been terminally acylated or alkylated;  
       f) p is an integer 0-3, with the provision that when X is halogen p equals O, when X is O p equals 1, when X is S p equals 1 or 2, and when X is N p equals 1, 2 or 3, and with the further provision that for p equals 2 or 3 the two or three groups R 2  may be identical or different;  
       g) R 3  is a straight, cyclic or branched alkyl, —O-alkyl, hydroxyalkyl, phenylalkyl, with up to 18 carbon atoms in the alkyl part, and R 4  is a straight, branched or cyclic alkyl group, such as C 1 -C 18  alkyl;  
       h) q is an integer >0 representing that one or more hydrogens in R 3  may have been replaced with a respective primary amino group (—NH 2 ) or a respective —NH—CR 4 ═O group.  
     
     
       2. A coated polymer article according to  claim 1 , wherein said basic polymer of the article is cross-linked polystyrene-divinylbenzene and L is a benzene ring. 
     
     
       3. A coated polymer article according to  claim 1 , wherein said article is in the form of particles with a diameter of 3-100 μm. 
     
     
       4. Method of performing chromatographic separation, said method comprising contacting the material to be separated with a separation medium comprising said polymer article according to  claim 1 . 
     
     
       5. Method of performing solid phase oligonucleotide synthesis, said method comprising synthesizing oligonucleotides on a solid support comprising said polymer article according to  claim 1 , exhibiting primary or secondary amino groups. 
     
     
       6. Method of performing reverse phase chromatography said method comprising contacting the material to be separated with a medium comprising said polymer article according to  claim 1 .