US6448077B1ExpiredUtility

Chimeric and humanized monoclonal antibodies specific to VEGF receptors

89
Assignee: IMCLONE SYSTEMS INCPriority: Feb 10, 1994Filed: Nov 10, 1997Granted: Sep 10, 2002
Est. expiryFeb 10, 2014(expired)· nominal 20-yr term from priority
C07K 2317/77A61K 38/00C07K 2317/73C07K 2317/24C07K 2319/00C07K 14/71C07K 2317/74C07K 16/2863
89
PatentIndex Score
53
Cited by
23
References
38
Claims

Abstract

Chimeric and humanized monoclonal antibodies that specifically bind to an extracellular domain of a VEGF receptor and neutralize activation of the receptor are provided. In vitro and in vivo methods of using these antibodies are also provided.

Claims

exact text as granted — not AI-modified
What is claimed is:  
     
       1. A monoclonal antibody that specifically binds to an extracellular domain of a flt-1 receptor and neutralizes activation of the receptor. 
     
     
       2. An antibody of  claim 1 , wherein the flt-1 receptor is mammalian. 
     
     
       3. An antibody of  claim 1 , wherein the flt-1 receptor is human. 
     
     
       4. A cell line producing a monoclonal antibody of  claim 1 . 
     
     
       5. A cell line producing the antibody of  claim 3 . 
     
     
       6. A composition comprising the antibody of  claim 1  or  claim 3  and a pharmaceutically acceptable carrier. 
     
     
       7. The composition of  claim 6  further comprising a chemotherapeutic agent and a pharmaceutically acceptable carrier. 
     
     
       8. A monoclonal antibody that specifically binds to an extracellular domain of a flt-1 receptor and reduces tumor growth. 
     
     
       9. An antibody of  claim 8 , wherein the flt-1 receptor is mammalian. 
     
     
       10. An antibody of  claim 8 , wherein the flt-1 receptor is human. 
     
     
       11. A cell line producing a monoclonal antibody of  claim 8 . 
     
     
       12. A cell line producing the antibody of  claim 10 . 
     
     
       13. A composition comprising the antibody of  claim 8  or  claim 10  and a pharmaceutically acceptable carrier. 
     
     
       14. The composition of  claim 13  further comprising a chemotherapeutic agent and a pharmaceutically acceptable carrier. 
     
     
       15. A humanized monoclonal antibody that specifically binds to an extracellular domain of a flt-1 receptor and reduces tumor growth. 
     
     
       16. An antibody of  claim 15 , wherein the flt-1 receptor is mammalian. 
     
     
       17. An antibody of  claim 15 , wherein the flt-1 receptor is human. 
     
     
       18. A cell line producing the humanized monoclonal antibody of  claim 15 . 
     
     
       19. A cell line producing the antibody of  claim 17 . 
     
     
       20. A composition comprising the antibody of  claim 15  or  claim 17  and a pharmaceutically acceptable carrier. 
     
     
       21. The composition of  claim 20  further comprising a chemotherapeutic agent and a pharmaceutically acceptable carrier. 
     
     
       22. A humanized monoclonal antibody that specifically binds to an extracellular domain of a flt-1 receptor and reduces tumor growth. 
     
     
       23. An antibody of  claim 22 , wherein the flt-1 receptor is mammalian. 
     
     
       24. An antibody of  claim 22 , wherein the flt-1 receptor is human. 
     
     
       25. A cell line producing the humanized monoclonal antibody of  claim 22 . 
     
     
       26. A cell line producing the antibody of  claim 24 . 
     
     
       27. A composition comprising the antibody of  claim 22  or  claim 24  and a pharmaceutically acceptable carrier. 
     
     
       28. The composition of  claim 27  further comprising a chemotherapeutic agent and a pharmaceutically acceptable carrier. 
     
     
       29. An antibody of  claim 1  or  claim 8 , wherein the antibody is chimerized. 
     
     
       30. A cell line of  claim 4  or  claim 11 , wherein the monoclonal antibody is chimerized. 
     
     
       31. A monoclonal antibody that specifically binds to an extracellular domain of a flt-1 receptor and inhibits angiogenesis. 
     
     
       32. A monoclonal antibody of  claim 31 , wherein the flt-1 receptor is mammalian. 
     
     
       33. A monoclonal antibody of  claim 31 , wherein the flt-1 receptor is human. 
     
     
       34. A monoclonal antibody of  claim 31 , wherein the antibody is chimerized. 
     
     
       35. A monoclonal antibody of  claim 31 , wherein the monoclonal antibody is humanized. 
     
     
       36. A cell line producing a monoclonal antibody of  claim 31 . 
     
     
       37. A composition comprising an antibody of  claim 31  and a pharmaceutically acceptable carrier. 
     
     
       38. A composition of  claim 37  further comprising a chemotherpeutic agent.

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