DNA fragment responsive to low temperatures and a plant transformed with the DNA fragment

Abstract

This invention relates to a DNA fragment comprising a base sequence (a) the base sequence referred to as nucleotide numbers 1-3794 in a sequence number 1 in a sequence list; (b) the base sequence (a) a part of which is deleted or substituted by another base sequence, or to which another base sequence is added. Further, the invention relates to a recombinant DNA and transformed plant including the above fragment. Moreover, the invention relates to fragment developing a promoter activity with a responsive property to low temperatures which comprises a base sequence (c) the base sequence referred to as nucleotide numbers 1-2797 in a sequence number 1 in a sequence list; (d) A part of the base sequence (c) developing a promoter activity with a responsive property to low temperatures; or (e) The base sequence (c) or (d) a part of which is deleted or substituted by another base sequence, or to which another base sequence is added. Further, the invention relates to a recombinant DNA and transformed plant including the above fragment.

What is claimed is:  
     
       1. An isolated DNA fragment comprising nucleotides 1-2797 of SEQ ID NO:1, wherein said DNA fragment is a promoter which is induced by low temperatures. 
     
     
       2. A recombinant DNA comprising the DNA fragment as claimed in  claim 1 . 
     
     
       3. A transformed plant expressing a specific protein in response to low temperatures, in which the transformed plant was transformed with a recombinant DNA comprising a nucleic acid encoding said protein operably linked to the DNA fragment of  claim 1 .