Threshold glucose detection in urine

Abstract

The present invention is directed to a reagent composition for use in detection and quantification of glucose in urine comprising a first chromogenic indicator reagent combination capable of color development to indicate the presence of a lower concentration of glucose, a second chromogenic indicator reagent combination capable of color development to indicate the presence of a higher concentration of glucose, wherein the first chromogenic indicator prevents color development of the second chromogenic indicator unless the higher concentration of glucose is present, a scavenger that prevents color development of the first indicator reagent composition unless a threshold concentration of glucose is present.

What is claimed is:  
     
       1. A reagent composition for use in detection and quantification of glucose in urine comprising: 
       a first chromogenic indicator reagent combination capable of color development to indicate the presence of a lower concentration of glucose, and  
       a second chromogenic indicator reagent combination capable of color development to indicate the presence of a higher concentration of glucose, wherein the first chromogenic indicator prevents color development of the second chromogenic indicator unless the higher concentration of glucose is present, and  
       a scavenger that prevents color development of the first indicator reagent composition unless a threshold concentration of glucose is present.  
     
     
       2. The composition of  claim 1  wherein the scavenger comprises cysteine. 
     
     
       3. The composition of  claim 1  wherein the scavenger is selected from the group consisting of thiosulfates and mercaptans. 
     
     
       4. The composition of  claim 1  wherein the threshold glucose concentration is about 50 mg/dL. 
     
     
       5. The dev composition ice of  claim 4  wherein the lower concentration of glucose is in the range of 150-300 mg/dL. 
     
     
       6. The composition of  claim 5  wherein the higher concentration of glucose is 300 mg/dL and the second indicator is capable of color development to indicate concentrations of glucose in the range of 300-600 mg/dL. 
     
     
       7. The composition of  claim 1  wherein the first indicator comprises 4-aminoantipyrene and a phenolic compound. 
     
     
       8. The composition of  claim 7  wherein the phenolic compound is selected from the group consisting of 2-hydroxy-3,5-dichlorobenzenesulfonate, 3-methylcatechol, 4-hydroxybenzenesulfonic acid, and 2,6-dimethylphenol. 
     
     
       9. The composition of  claim 1  wherein the second indicator comprises an iodide salt. 
     
     
       10. The composition of  claim 9  wherein the iodide salt is potassium iodide and the second indicator further comprises starch. 
     
     
       11. The composition of  claim 1  wherein a resultant color obtained from the color development is independent of the specific gravity of the urine.