US6602693B1ExpiredUtility

Gene encoding hyaluronan synthase

27
Assignee: CLEAR SOLUTIONS BIOTECH INCPriority: Jul 3, 1996Filed: Mar 5, 1997Granted: Aug 5, 2003
Est. expiryJul 3, 2016(expired)· nominal 20-yr term from priority
Inventors:John McdonaldAndrew Pearce SpicerMary Louise Augustine
A61K 38/00C12N 9/1051
27
PatentIndex Score
2
Cited by
13
References
24
Claims

Abstract

An isolated and purified DNA molecule encoding hyaluronan synthase-2 (Has2) is provided, as is purified and isolated Has2 polypeptide. Also provided is an isolated and purified DNA molecule encoding hyaluronan synthase-3 (Has3), as is purified and isolated Has3 polypeptide.

Claims

exact text as granted — not AI-modified
What is claimed is:  
     
       1. An isolated and purified DNA molecule comprising a mammalian DNA segment encoding a polypeptide having hyaluronan synthase activity, or the complement thereof, wherein the DNA segment hybridizes under hybridizing conditions to SEQ ID NO:31 or the complement thereof, and wherein the DNA segment remains hybridized to SEQ ID NO:31 or the complement thereof under stringent wash conditions at a temperature of 50° C. or greater but lower than the Tm in 0.1×SSC. 
     
     
       2. The DNA molecule of  claim 1  wherein the DNA segment encodes a murine hyaluronan synthase. 
     
     
       3. The DNA molecule of  claim 1  wherein the DNA segment encodes a hyaluronan synthase having SEQ ID NO:32. 
     
     
       4. The DNA molecule of  claim 1  wherein the DNA segment comprises SEQ ID NO:31. 
     
     
       5. The DNA molecule of  claim 1  wherein the DNA segment encodes a human hyaluronan synthase. 
     
     
       6. The DNA molecule of  claim 5  wherein the DNA segment comprises SEQ ID NO:25. 
     
     
       7. The DNA molecule of  claim 5  wherein the DNA segment encodes a polypeptide comprising SEQ ID NO29. 
     
     
       8. An expression cassette comprising a promoter operably linked to a DNA segment encoding a polypeptide having hyaluronan synthase activity or the complement thereof, wherein the DNA segment hybridizes under hybridizing conditions to SEQ ID NO:31 or the complement thereof, and wherein the DNA segment remains hybridized to SEQ ID NO:31 or the complement thereof under stringent wash conditions at a temperature of 50° C. or greater but lower than the Tm in 0.1×SSC. 
     
     
       9. The expression cassette of  claim 8  wherein the DNA segment encodes a murine hyaluronan synthase. 
     
     
       10. The expression cassette of  claim 8  wherein the DNA segment encodes a hyaluronan synthase having SEQ ID NO:32. 
     
     
       11. The expression cassette of  claim 8  wherein the DNA segment comprises SEQ ID NO:31. 
     
     
       12. The expression cassette of  claim 8  wherein the DNA segment encodes a human hyaluronan synthase. 
     
     
       13. The expression cassette of  claim 12  wherein the DNA segment comprises SEQ ID NO:25. 
     
     
       14. A host cell, the genome of which is augmented by a DNA segment encoding a polypeptide having hyaluronan synthase activity or the complement thereof, wherein the DNA segment hybridizes under hybridizing conditions to SEQ ID NO:31 or the complement thereof, and wherein the DNA segment remains hybridized to SEQ ID NO:31 or the complement thereof under stringent wash conditions at a temperature of 50° C. or greater but lower than the Tm in 0.1×SSC. 
     
     
       15. The host cell of  claim 14  wherein the DNA segment encodes a murine hyaluronan synthase. 
     
     
       16. The host cell of  claim 14  wherein the DNA segment encodes a hyaluronan synthase having SEQ ID NO:32. 
     
     
       17. The host cell of  claim 14  wherein the DNA segment encodes a human hyaluronan synthase. 
     
     
       18. A method to produce hyaluronan synthase, comprising: culturing a host cell transformed with a nucleic acid molecule comprising a promoter operably linked to a DNA segment encoding a polypeptide having hyaluronan synthase activity, so that said host cell expresses said hyaluronan synthase, wherein the DNA segment hybridizes under hybridizing conditions to SEQ ID NO:31 or the complement thereof, and wherein the DNA segment remains hybridized to SEQ ID NO:31 or the complement thereof under stringent wash conditions at a temperature of 50° C. or greater but lower than the Tm in 0.1×SSC. 
     
     
       19. The method of  claim 18  further comprising isolating hyaluronan synthase from the host cell. 
     
     
       20. The method of  claim 18  wherein the DNA segment encodes a murine hyaluronan synthase. 
     
     
       21. The method of  claim 20  wherein the DNA segment comprises SEQ ID NO:31. 
     
     
       22. The method of  claim 18  wherein the DNA segment encodes a hyaluronan synthase having SEQ ID NO:32. 
     
     
       23. The method of  claim 18  wherein the DNA segment encodes a human hyaluronan synthase. 
     
     
       24. The method of  claim 23  wherein the DNA segment comprises SEQ ID NO:25.

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