Nucleotide sequences which code for the zwa1 gene

Abstract

The invention relates to an isolated polynucleotide comprising a polynucleotide sequence chosen from the group consisting of</PTEXT>a) polynucleotide which is identical to the extent of at least 70% to a polynucleotide which codes for a polypeptide which comprises the amino acid sequence of SEQ ID NO 2,</PTEXT>b) polynucleotide which codes for a polypeptide which comprises an amino acid sequence which is identical to the extent of at least 70% to the amino acid sequence of SEQ ID NO 2</PTEXT>c) polynucleotide which is complementary to the polynucleotides of a) and b) and</PTEXT>d) polynucleotide comprising at least 15 successive nucleotides of the polynucleotide sequence of a), b) or c),</PTEXT>and processes for the fermentative preparation of L-amino acid with amplification of the zwa1 gene in the coryneform bacteria employed.</PTEXT>

We claim:  
     
       1. A process for the preparation of L-lysine comprising: 
       culturing a Coryneform bacteria, comprising an overexpressed zwa1 gene having the polynucleotide sequence of SEQ ID NO: 1, in a medium suitable for expression of the zwa1 gene to thereby produce L-lysine.  
     
     
       2. The process according to  claim 1 , and further comprising: 
       concentrating the L-lysine produced in the medium or in the cells of the bacteria.  
     
     
       3. The process according to  claim 2 , and further comprising: 
       isolating the L-lysine.  
     
     
       4. The process according to  claim 1 , wherein, said Coryneform bacteria further comprises one or more genes selected from the group consisting of 
       1) the dapA gene which codes for dihydrodipicolinate synthase,  
       2) the lysC gene which codes for a feed back resistant aspartate kinase,  
       3) the dapD gene which codes for tetradihydrodipicolinate succinylase,  
       4) the dapE gene which codes for succinyl diaminopimelate desuccinylase,  
       5) the gap gene which codes for glyceraldehyde 3-phosphate dehydrogenase,  
       6) the pyc gene which codes for pyruvate carboxylase,  
       7) the mqo gene which codes for malate:quinone oxidoreductase, and  
       8) the lysE gene which codes for lysine export, which is/are overexpressed.  
     
     
       5. The process according to  claim 1 , wherein said Coryneform bacteria further comprises one or more genes selected from the group consisting of 
       1) the pck gene which codes for phosphoenol pyruvate carboxykinase; and  
       2) the pgi gene which codes for glucose 6-phosphate isomerase which is/are attenuated.  
     
     
       6. The process according to  claim 1 , wherein said Coryneform bacterium is of the species  Corynebacterium glutamicum.    
     
     
       7. The process according to  claim 1 , wherein said polynucleotide sequence includes nucleotides 413 to 1009 of SEQ ID NO: 1. 
     
     
       8. A process for producing L-lysine comprising: 
       a) transforming a Coryneform bacterium with a vector comprising a zwa1 gene having the polynucleotide sequence of SEQ ID NO: 1, wherein said zwa1 gene is under the control of a promoter which allows the over expression of said zwa1 gene;  
       b) culturing said Coryneform bacterium in a medium suitable for expression of the zwa1 gene to thereby produce L-lysine; and  
       c) isolating the L-lysine.  
     
     
       9. The process of  claim 8  wherein the vector is pCR2.1zwa1exp deposited under the number DSM 13115. 
     
     
       10. A vector pCR2.1zwa1exp deposited under the number DSM 13115.