High-density functional slide and preparation method thereof

Abstract

The invention features a method for preparing a high-density functional slide by coating a sol-gel containing amine-group bearing silanes and a solution containing polyaldehyde groups onto an organic or inorganic substrate, respectively. The resulting slide is useful in the preparation of highly homogeneous functional-group slides and the high-density and high-efficiency bio-chip/microarray.

What is claimed is:  
     
       1. A microarray having high-density functional groups for immobilization of a bio-molecule, comprising: 
       (i) a substrate;  
       (ii) an interlayer of a silane formed by coating a sol-gel of silanes onto said substrate;  
       (iii) a polyaldehyde layer formed onto said interlayer; and  
       (iv) a biologically active material, which is immobilized onto said polyaldehyde layer.  
     
     
       2. The microarray as claimed in  claim 1 , wherein the substrate comprises an organic or inorganic substrate. 
     
     
       3. The microarray as claimed in  claim 2 , wherein the organic substrate comprises a polymer polymerized by organic monomers, wherein said organic monomers are selected from the group consisting of a monomer of ethylene, propylene, ester, acrylic acid, acrylate, alkyl acrylic acid, and alkyl acrylate. 
     
     
       4. The microarray as claimed in  claim 2 , wherein the inorganic substrate is selected from the group consisting of silicon wafer, ceramic material, glass, and metal. 
     
     
       5. The microarray as claimed in  claim 1 , wherein said sol-gel of silanes is polymerized by a monomer of silane in the presence of a catalyst. 
     
     
       6. The microarray as claimed in  claim 5 , wherein the monomer comprises silane with amine group. 
     
     
       7. The microarray as claimed in  claim 5 , wherein the monomer comprises mixtures of silane with and without an amine group. 
     
     
       8. The microarray as claimed in  claim 7 , wherein the mixtures of silane comprise an amine density that is controlled by mixing varying ratios of silane with an amine group to silane without an amine group. 
     
     
       9. The microarray as claimed in  claim 8 , wherein the amine groups on said interlayer are covalently bonded to part of the aldehyde groups on said polyaldehyde layer. 
     
     
       10. The microarray as claimed in  claim 9 , wherein part of the aldehyde groups on said polyaldehyde layer are unbonded to the amine groups on said interlayer, said unbonded aldehyde groups being exposed to form a functional layer on said microarray with a distribution of high density aldehyde groups. 
     
     
       11. The microarray as claimed in  claim 10 , wherein the unbonded aldehyde groups on the microarray are bonded to an amine-group of a bio-molecule to be immobilized by forming a Schiff linkage. 
     
     
       12. The microarray as claimed in  claim 7 , wherein the monomer of silane with an amine group comprises aminopropyltriethoxysilane (APTES), and the monomer of silane without an amine group comprises tetraethoxysilane (TEOS). 
     
     
       13. The microarray as claimed in  claim 1 , wherein the polyaldehyde comprises a polyvinylalcohol-based polyaldehyde graft copolymer. 
     
     
       14. The microarray as claimed in  claim 13 , wherein the polyvinylalcohol-based polyaldehyde graft copolymer is prepared by co-polymerization of allyl alcohol and acrolein using polyvinylalcohol as a stem nucleus. 
     
     
       15. The microarray as claimed in  claim 14 , wherein the polyvinylalcohol-based polyaldehyde graft copolymer comprises an aldehyde density that is controlled by mixing different ratios of allyl alcohol to acrolein. 
     
     
       16. The microarray as claimed in  claim 1 , wherein the biologically active material comprises nucleic acid, oligonucleotide, peptide, peptide nucleic acid, antigen, antibody, enzyme, or protein. 
     
     
       17. The microarray as claimed in  claim 1 , wherein said microarray is characterized by the decreasing the steric hindrance and promoting the efficiencies of immobilization and hybridization during the immobilization of oligonucleotide probes and the analysis of sequences.