US6743774B1ExpiredUtility

Tribonectins

90
Assignee: RHODE ISLAND HOSPITALPriority: Apr 23, 1999Filed: Apr 23, 1999Granted: Jun 1, 2004
Est. expiryApr 23, 2019(expired)· nominal 20-yr term from priority
Inventors:Gregory D. Jay
A61P 43/00A61P 41/00A61P 9/00A61K 38/00A61P 19/02A61K 38/1709A61P 17/02C07K 14/47A61K 48/00A61P 19/10C07K 14/475
90
PatentIndex Score
56
Cited by
35
References
26
Claims

Abstract

The invention features a tribonectin and a method of tribosupplementation carried out by administering tribonectins directly to an injured or arthritic joint.

Claims

exact text as granted — not AI-modified
What is claimed is:  
     
       1. A method of lubricating a mammalian joint, comprising contacting said joint with a purified polypeptide comprising residues 200-1140 of SEQ ID NO:1 and an O-linked oligosaccharide. 
     
     
       2. The method of  claim 1 , wherein said joint is an articulating joint of a human. 
     
     
       3. The method of  claim 1 , wherein said joint is an articulating joint of a dog. 
     
     
       4. The method of  claim 1 , wherein said joint is an articulating joint of a horse. 
     
     
       5. The method of  claim 1 , wherein said tribonectin is administered intra-articularly. 
     
     
       6. A method of inhibiting adhesion formation between a first surface and a second surface in a mammal, said method comprising placing a tribonectin between said first and second surfaces in an amount sufficient to prevent adhesion of said surfaces in said mammal, wherein said tribonectin is a purified polypeptide comprising residues 200-1140 of SEQ ID NO:1 and an O-linked oligosaccharide. 
     
     
       7. The method of  claim 6 , wherein said first surface and said second surface are both injured tissues of said mammal. 
     
     
       8. The method of  claim 6 , wherein said first or said second surface is an artificial device. 
     
     
       9. The method of  claim 8 , wherein said artificial device is an orthopedic implant. 
     
     
       10. The method of  claim 6 , wherein said tribonectin is present in a composition, said composition being in the form of a membrane, foam, gel, or fiber. 
     
     
       11. The method of  claim 6 , wherein said first and said second surfaces are tissues injured due to a surgical incision. 
     
     
       12. The method of  claim 6 , wherein said first and said second surfaces are tissues injured due to trauma. 
     
     
       13. A method of lubricating a mammalian joint, comprising contacting said joint with a purified tribonectin, wherein said tribonectin is a fragment of megakaryocyte stimulating factor (MSF) comprising residues 200-1140 of SEQ ID NO: 1, wherein said fragment comprises an O-linked oligosaccharide. 
     
     
       14. The method of  claim 13 , wherein said tribonectin further comprises residues 1141-1167 of SEQ ID NO:1. 
     
     
       15. The method of  claim 14 , wherein said tribonectin further comprises residues 1168-1212 of SEQ ID NO: 1. 
     
     
       16. The method of  claim 15 , wherein said tribonectin further comprises residues 1213-1263 of SEQ ID NO: 1. 
     
     
       17. The method of  claim 13 , wherein said tribonectin comprises residues 200-1263 of SEQ ID NO: 1. 
     
     
       18. The method of  claim 1 , wherein said polypeptide is recombinant. 
     
     
       19. The method of  claim 6 , wherein said polypeptide is recombinant. 
     
     
       20. The method of  claim 1 , wherein said polypeptide is chemically synthesized. 
     
     
       21. The method of  claim 6 , wherein said polypeptide is chemically synthesized. 
     
     
       22. The method of  claim 1 ,  6 , or  13 , wherein said O-linked oligosaccharide is β(1,3)Gal-GalNAC. 
     
     
       23. The method of  claim 1 ,  6 , or  13 , wherein said O-linked oligosaccharide is β(1,3)Gal-GalNeuAc. 
     
     
       24. A method of lubricating a mammalian joint, comprising contacting said joint with a purified polypeptide comprising an amino acid sequence in which at least one threonine in the amino acid sequence of residues 200-1140 of SEQ ID NO:1 is substituted with a serine, wherein said polypeptide is glycosylated. 
     
     
       25. The method of  claim 24 , wherein said polypeptide comprises an O-linked oligosaccharide. 
     
     
       26. A method of lubricating a mammalian joint, comprising contacting said joint with a purified tribonectin, wherein said tribonectin is an alternatively spliced variant of human megakaryocyte stimulating factor (MSF) gene, wherein said variant comprises at least an O-glycosylated mucin domain, wherein said mucin domain is encoded by exon 6 of a human MSF gene.

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