14189, a novel human kinase and uses thereof

Abstract

A novel human kinase protein and nucleic acid molecule is disclosed. In addition to the isolated kinase protein, the invention further provides isolated kinase fusion proteins, antigenic peptides, and anti-kinase antibodies. The invention also provides kinase nucleic acid molecules, recombinant expression vectors containing a nucleic acid molecule of the invention, host cells into which the expression vectors have been introduced, and nonhuman transgenic animals in which a kinase gene has been introduced or disrupted. Diagnostic, screening, and therapeutic methods utilizing compositions of the invention are also provided.

What is claimed is:  
     
       1. An isolated nucleic acid molecule selected from the group consisting of: 
       a) a nucleic acid molecule comprising a nucleotide sequence that is at least 97% identical to the nucleotide sequence of SEQ ID NO: 1, at least 97% identical to nucleotides 48-1682 of SEQ ID NO: 1, or at least 97% identical to the cDNA insert of the plasmid deposited with ATCC as Accession Number PTA-2333, wherein the nucleic acid molecule encodes a polypeptide with kinase activity;  
       b) a nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO: 1, nucleotides 48-1682 of SEQ ID NO: 1, or the cDNA insert of the plasmid deposited with ATCC as Accession Number PTA-2333, wherein the nucleic acid molecule encodes a polypeptide with kinase activity;  
       c) a nucleic acid molecule which encodes a polypeptide comprising the amino acid sequence of SEQ ID NO: 2, or the amino acid sequence encoded by the cDNA insert of the plasmid deposited with ATCC as Accession Number PTA-2333, wherein the nucleic acid molecule encodes a polypeptide with kinase activity; and  
       d) a nucleic acid molecule which comprises the complement of the nucleic acid molecule of a), b) or c).  
     
     
       2. The nucleic acid molecule of  claim 1  further comprising nucleic acid sequences encoding a heterologous polypeptide. 
     
     
       3. A kit comprising a nucleic acid molecule of  claim 1  and instructions for use. 
     
     
       4. A host cell genetically engineered to contain the nucleic acid molecule of  claim 1 . 
     
     
       5. A host cell genetically engineered to contain the nucleic acid molecule of  claim 2 . 
     
     
       6. A host cell genetically engineered to express the nucleic acid molecule of  claim 1 . 
     
     
       7. A host cell genetically engineered to express the nucleic acid molecule of  claim 2 . 
     
     
       8. The host cell of  claim 4  which is a mammalian host cell. 
     
     
       9. The host cell of  claim 5  which is a mammalian host cell. 
     
     
       10. The host cell of  claim 6  which is a mammalian host cell. 
     
     
       11. The host cell of  claim 7  which is a mammalian host cell. 
     
     
       12. A vector comprising the nucleic acid molecule of  claim 1 . 
     
     
       13. A vector comprising the nucleic acid molecule of  claim 2 . 
     
     
       14. The vector of  claim 12  further comprising a nucleic acid sequence which regulates expression of the nucleic acid molecule. 
     
     
       15. The vector of  claim 13  further comprising a nucleic acid sequence which regulates expression of the nucleic acid molecule. 
     
     
       16. A host cell comprising the vector of  claim 12 . 
     
     
       17. A host cell comprising the vector of  claim 13 . 
     
     
       18. The host cell of  claim 16  which is a mammalian host cell. 
     
     
       19. The host cell of  claim 17  which is a mammalian host cell. 
     
     
       20. A method of producing a polypeptide comprising: culturing the host cell of  claim 6  under conditions in which the nucleic acid molecule is expressed to produce said polypeptide. 
     
     
       21. A method of producing a polypeptide comprising: culturing the host cell of  claim 7  under conditions in which the nucleic acid molecule is expressed to produce said polypeptide. 
     
     
       22. An isolated nucleic acid molecule selected from the group consisting of: 
       a) a nucleic acid molecule consisting of the nucleotide sequence of SEQ ID NO: 1, nucleotides 48-1682 of SEQ ID NO: 1, or the cDNA insert of the plasmid deposited with ATCC as Accession Number PTA-2333, wherein the nucleic acid molecule encodes a polypeptide with kinase activity; and  
       b) a nucleic acid molecule which comprises the complement of the nucleic acid molecule of a).