Non-nucleosidic coumarin derivatives as polynucleotide-crosslinking agents

Abstract

Novel coumarin derivatives comprising a coumarin moiety linked to a non-nucleosidic backbone moiety are disclosed. The resulting molecules are typically used as photoactivate crosslinking groups when incorporated into polynucleotides as replacements for one or more of the complementary nucleoside bases present in probes used in procedures involving nucleic acid hybridization reactions.

We claim:  
     
       1. A compound having the formula:                    
     
     
       2. A compound having the formula:                    
     
     
       3. A compound having the formula:                    
     
     
       4. A compound having the formula:                    
     
     
       5. A compound having the formula:                    
       wherein P 1  and P 2 , independently, are protecting groups. 
     
     
       6. The compound of  claim 5 , wherein P 1  is a trityl. 
     
     
       7. The compound of  claim 5 , wherein P 2  is a phosphoramidite. 
     
     
       8. The compound of  claim 5 , wherein P 1  is a trityl and P 2  is a phosphoramidite. 
     
     
       9. A compound having the formula:                    
       wherein P 1  and P 2 , independently, are protecting groups. 
     
     
       10. The compound of  claim 9 , wherein P 1  is a trityl. 
     
     
       11. The compound of  claim 9 , wherein P 2  is a phosphoramidite. 
     
     
       12. The compound of  claim 9 , wherein P 1  is a trityl and P 2  is a phosphoramidite. 
     
     
       13. A compound having the formula:                    
       wherein P 1  and P 2 , independently, are protecting groups. 
     
     
       14. The compound of  claim 13 , wherein P 1  is a trityl. 
     
     
       15. The compound of  claim 13 , wherein P 2  is a phosphoramidite. 
     
     
       16. The compound of  claim 13 , wherein P 1  is a trityl and P 2  is a phosphoramidite. 
     
     
       17. A compound having the formula:                    
       wherein P 1  and P 2 , independently, are protecting groups. 
     
     
       18. The compound of  claim 17 , wherein P 1  is a trityl. 
     
     
       19. The compound of  claim 17 , wherein P 2  is a phosphoramidite. 
     
     
       20. The compound of  claim 17 , wherein P 1  is a trityl and P 2  is a phosphoramidite. 
     
     
       21. A compound having the formula:                    
       wherein one of P 1  and P 2  is a protecting group and the other is a hydrogen atom. 
     
     
       22. The compound of  claim 21 , wherein P 1  is a trityl. 
     
     
       23. The compound of  claim 21 , wherein P 2  is a phosphoramidite. 
     
     
       24. A compound having the formula:                    
       wherein one of P 1  and P 2  is a protecting group and the other is a hydrogen atom. 
     
     
       25. The compound of  claim 24 , wherein P 1  is a trityl. 
     
     
       26. The compound of  claim 24 , wherein P 2  is a phosphoramidite. 
     
     
       27. A compound having the formula:                    
       wherein one of P 1  and P 2 , is a protecting group and the other is a hydrogen atom. 
     
     
       28. The compound of  claim 27 , wherein P 1  is a trityl. 
     
     
       29. The compound of  claim 27 , wherein P 2  is a phosphoramidite. 
     
     
       30. A compound having the formula:                    
       wherein one of P 1  and P 2 , is a protecting group and the other is a hydrogen atom. 
     
     
       31. The compound of  claim 30 , wherein P 1  is a trityl. 
     
     
       32. The compound of  claim 30 , wherein P 2  is a phosphoramidite.