Nematode PGM-like sequences

Abstract

Disclosed is a nucleic acid molecule from nematodes encoding for phosphoglycerate mutase (PGM) polypeptides. The PGM-like polypeptide sequence is also provided, as are vectors, host cells, and recombinant methods for production of PGM-like nucleotides and polypeptides. The invention further relates to screening methods for identifying inhibitors and/or activators, as well as methods for antibody production. Such inhibitors are useful for control nematode infection.

What is claimed is:  
     
       1. An isolated nucleic acid molecule encoding a polypeptide comprising an amino acid sequence that is at least 95% identical to the amino acid sequence of SEQ ID NO:2, wherein the polypeptide has phosphoglycerate mutase activity. 
     
     
       2. The isolated nucleic acid molecule of  claim 1  wherein the amino acid sequence is at least 98% identical to the amino acid sequence of SEQ ID NO:2. 
     
     
       3. An isolated nucleic acid molecule comprising the nucleotide sequence of nucleotides 22 to 1599 of SEQ ID NO: 1. 
     
     
       4. The isolated nucleic acid molecule of  claim 1 , further comprising a heterologous promoter operably linked to the isolated nucleic acid. 
     
     
       5. An isolated nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO: 1. 
     
     
       6. A vector comprising the isolated nucleic acid molecule of any one of claims  1 ,  2 ,  3 ,  4  and  5 . 
     
     
       7. The vector of  claim 6  further comprising a heterologous promoter operably linked to the isolated nucleic acid molecule. 
     
     
       8. An isolated host cell comprising the isolated nucleic acid molecule of any one of claims  1 ,  2 ,  3 ,  4  and  5 . 
     
     
       9. An isolated host cell comprising the vector of  claim 6  . 
     
     
       10. An isolated host cell comprising the vector of  claim 7 .