Method of isolation and purification of trypsin from pronase protease and use thereof

Abstract

The present invention is directed to methods of isolation and purification of Streptomyces griseus trypsin (SGT) from PRONASE protease in a single affinity chromatography step and uses of the purified SGT.

What is claimed is:  
     
       1. A purified preparation of  Streptomyces griseus trypsin  (SGT) having a specific activity of at least about 25×10 3  U/mg protein and a purity of at least 95% and further comprising arginine. 
     
     
       2. The preparation of purified SGT according to  claim 1 , wherein said arginine has a concentration of at least about 0.5 M. 
     
     
       3. A purified preparation of  Streptomyces griseus trypsin  (SGT) having a specific activity of at least about 25×10 3  U/mg protein and a purity of at least 95%, wherein said preparation is obtained by a method comprising contacting PRONASE protease with an immobilized affinity moiety comprising benzamidine and eluting said SGT selectively from said immobilized affinity moiety with an eluant comprising arginine.