US6893642B1ExpiredUtility

Algae protein polysaccharide extraction and use thereof

74
Priority: Jul 19, 1999Filed: Jul 19, 2000Granted: May 17, 2005
Est. expiryJul 19, 2019(expired)· nominal 20-yr term from priority
Inventors:Qing QiJian Ding
A61K 38/168A61P 37/02A61P 37/04A61P 7/00A61P 31/12Y10S435/946A61P 43/00A61P 35/00
74
PatentIndex Score
7
Cited by
6
References
54
Claims

Abstract

The present invention provides a pharmaceutical composition containing proteoglycan extracts of algae and a process for preparing proteoglycan extracts from the algae.

Claims

exact text as granted — not AI-modified
1. An anticancer composition comprising a therapeutically effective amount of proteoglycan extract from Spirulina with or without a pharmaceutically acceptable carrier, wherein the proteoglycan extract from Spirulina is prepared by the following steps of:
 a) dissolving a dry powdered Spirulina in 5-20 times water by weight and breaking the Spirulina cell walls;  
 b) heating a solution obtained from step a) at 60°-100° C., and cooling the heated solution to separate a liquid phase from the solution;  
 c) adjusting pH of said liquid phase to 3.8-4.2, and filtering said liquid phase to obtain a filtrate; and  
 d) adjusting the filtrate to pH 7, and concentrating the filtrate.  
 
     
     
       2. The composition according to  claim 1 , wherein said water in step a) is 8-15 times by weight of said dry powdered Spirulina. 
     
     
       3. The composition according to  claim 3 , wherein said water in step a) is 10 times by weight of said dry powdered Spirulina. 
     
     
       4. The composition according to  claim 1 , wherein said step b) is conducted at a temperature of 80° C.-95° C. 
     
     
       5. The composition according to  claim 4 , wherein step b) is conducted at a temperature of 90° C. 
     
     
       6. A hemogram-improving composition comprising a therapeutically effective amount of proteoglycan extract from Spirulina with or without a pharmaceutically acceptable carrier, wherein the proteoglycan extract from Spirulina is prepared by the following steps of:
 a) dissolving a dry powdered Spirulina in 5-20 times water by weight, and breaking the Spirulina cell walls;  
 b) heating a solution obtained from step a) at 60°-100° C., and cooling the heated solution to separate a liquid phase from the solution;  
 c) adjusting pH of said liquid phase to 3.8-4.2, and filtering said liquid phase to obtain a filtrate; and  
 d) adjusting the filtrate to pH 7, and concentrating the filtrate.  
 
     
     
       7. The composition according to  claim 6 , wherein said water in step a) is 8-15 times by weight of said dry powdered Spirulina. 
     
     
       8. The composition according to  claim 7 , wherein said water in step a) is 10 times by weight of said dry powdered Spirulina. 
     
     
       9. The composition according to  claim 6 , wherein said step b) is conducted at a temperature of 80° C.-95° C. 
     
     
       10. The composition according to  claim 9  wherein said step b) is conducted at a temperature of 90° C. 
     
     
       11. An anti-irradiation composition comprising a therapeutically effective amount of proteoglycan extract from Spirulina with or without a pharmaceutically acceptable carrier, wherein the proteoglycan extract from spirulina is prepared by the following steps of:
 a) dissolving a dry powdered Spirulina in 5-20 times water by weight, and breaking the Spirulina cell walls;  
 b) heating a solution obtained from step a) at 60°-100° C., and cooling the heated solution to separate a liquid phase from the solution;  
 c) adjusting pH of said liquid phase to 3.8-4.2, and filtering said liquid phase to obtain a filtrate; and  
 d) adjusting the filtrate to pH 7, and concentrating the filtrate.  
 
     
     
       12. The composition according to  claim 11 , wherein said water in step a) is 8-15 tunes by weight of said dry powdered Spirulina. 
     
     
       13. The composition according to  claim 12 , wherein said water in step a) is 10 times by weight of said dry powdered Spirulina. 
     
     
       14. The composition according to  claim 11 , wherein said step b) is conducted at a temperature of 80° C.-95° C. 
     
     
       15. The composition according to  claim 14 , wherein said step b) is conducted at a temperature of 90° C. 
     
     
       16. A DNA-repairing composition comprising a therapeutically effective amount of proteoglycan extract from Spirulina with or without a pharmaceutically acceptable carrier, wherein the proteoglycan extract from Spirulina is prepared by the following steps of:
 a) dissolving a dry powdered Spirulina in 5-20 times water by weight, and breaking the Spirulina cell walls;  
 b) heating a solution obtained from step a) at 60°-100° C., and cooling the heated solution to separate a liquid phase from the solution;  
 c) adjusting pH of said liquid phase to 3.8-4.2, and filtering said liquid phase to obtain a filtrate; and  
 d) adjusting the filtrate to pH 7, and concentrating the filtrate.  
 
     
     
       17. The composition according to  claim 16 , wherein said water in step a) is 8-15 times by weight of said dry powdered Spirulina. 
     
     
       18. The composition according to  claim 17 , wherein said water in step a) is 10 times by weight of said dry powdered Spirulina. 
     
     
       19. The composition according to  claim 16 , wherein said step b) is conducted at a temperature of 80° C.-95° C. 
     
     
       20. The composition according to  claim 19 , wherein said step b) is conducted at a temperature of 90° C. 
     
     
       21. An antivirus composition comprising a therapeutically effective amount of proteoglycan extract from Spirulina with or without a pharmaceutically acceptable carrier, wherein the proteoglycan extract from Spirulina is prepared by the following steps of:
 a) dissolving a dry powdered Spirulina in 5-20 times water by weight, and breaking the Spirulina cell walls;  
 b) heating a solution obtained from step a) at 60°-100° C., and cooling the heated solution to separate a liquid phase from the solution;  
 c) adjusting pH of said liquid phase to 3.8-4.2, and filtering said liquid phase to obtain a filtrate; and  
 d) adjusting the filtrate to pH 7, concentrating, and drying if necessary.  
 
     
     
       22. The composition according to  claim 21 , wherein said water in step a) is 8-15 times by weight of said dry powdered Spirulina. 
     
     
       23. The composition according to  claim 22 , wherein said water in step a) is 10 times by weight of said dry powdered Spirulina. 
     
     
       24. The composition according to  claim 21 , wherein said step b) is conducted at a temperature of 80° C.-95° C. 
     
     
       25. The composition according to  claim 24 , wherein said step b) is conducted at a temperature of 90° C. 
     
     
       26. An immunoenhancing composition comprising a therapeutically effective amount of proteoglycan extract from Spirulina with or without a pharmaceutically acceptable carrier, wherein the proteoglycan extract from Spirulina is prepared by the following steps of:
 a) dissolving a dry powdered Spirulina in 5-20 times water by weight; and breaking the Spirulina cell walls;  
 b) heating a solution obtained from step a) at 60°-100° C., and cooling the heated solution to separate a liquid phase from the solution;  
 c) adjusting pH of said liquid phase to 3.8-4.2, and filtering said liquid phase to obtain a filtrate; and  
 d) adjusting the filtrate to pH 7, and concentrating the filtrate.  
 
     
     
       27. The composition according to  claim 26 , wherein said water in step a) is 8-15 times by weight of said dry powdered Spirulina. 
     
     
       28. The composition according to  claim 27 , wherein said water in step a) is 10 times by weight of said dry powdered Spirulina. 
     
     
       29. The composition according to  claim 26 , wherein said step b) is conducted at a temperature of 80° C.-95° C. 
     
     
       30. The composition according to  claim 29 , wherein said step b) is conducted at a temperature of 90° C. 
     
     
       31. A dendrite-like-cell-activating composition comprising a therapeutically effective amount of proteoglycan extract from Spirulina with or without a pharmaceutically acceptable carrier, wherein the proteoglycan extract from Spirulina is prepared by the following steps of
 a) dissolving a dry powdered Spirulina in 5-20 times water by weight, and breaking the Spirulina cell walls;  
 b) heating a solution obtained from step a) at 60°-100° C., and cooling the heated solution to separate a liquid phase from the solution;  
 c) adjusting pH of aid liquid phase to 3.8-4.2, and filtering said liquid phase to obtain a filtrate; and  
 d) adjusting the filtrate to pH 7, and concentrating the filtrate.  
 
     
     
       32. The composition according to  claim 31 , wherein said water in step a) is 8-15 times by weight of said dry powdered Spirulina. 
     
     
       33. The composition according to  claim 32 , wherein said water in step a) is 10 times by weight of said dry powdered Spirulina. 
     
     
       34. The composition according to  claim 31 , wherein said step b) is conducted at a temperature of 80° C.-95° C. 
     
     
       35. The composition according to  claim 34 , wherein said step b) is conducted at a temperature of 90° C. 
     
     
       36. A process for preparing a proteoglycan extract from Spirulina, including the following steps of:
 a) dissolving a dry powdered Spirulina in 5-20 times water by weight and breaking the Spirulina cell walls;  
 b) heating a solution obtained from step a) at 60°-00° C., and cooling the heated solution to separate a liquid phase from the solution;  
 c) adjusting pH of said liquid phase to 3.8-4.2, and filtering said liquid phase to obtain a filtrate; and  
 d) adjusting the filtrate to pH 7, and concentrating the filtrate.  
 
     
     
       37. The process according to  claim 36 , wherein said water in step a) is 8-15 times weight of said dry powdered Spirulina. 
     
     
       38. The process according to  claim 37 , wherein said water in step a) is 10 times by weight of said dry powdered Spirulina. 
     
     
       39. The process according to  claim 36 , wherein step b) is conducted at a temperature of 80° C.-95° C. 
     
     
       40. The process according to  claim 39 , wherein said step b) is conducted at a temperature of 90° C. 
     
     
       41. A composition comprising a proteoglycan extract from Spirulina with or without a pharmaceutically acceptable carrier, wherein the proteoglycan extract from Spirulina is prepared by the following steps of:
 a) dissolving a dry powdered Spirulina in 5-20 times water by weight, and breaking the Spirulina cell walls;  
 b) heating a solution obtained from step a) at 60°-100° C., and cooling the heated solution to separate a liquid phase from the solution;  
 c) adjusting pH of said liquid phase to 3.8-4.2, and filtering said liquid phase to obtain a filtrate; and  
 d) adjusting the filtrate to pH 7, and concentrating the filtrate.  
 
     
     
       42. The composition according to  claim 41 , wherein said water in step a) is 8-15 times by weight of said dry powdered Spirulina. 
     
     
       43. The composition according to  claim 42 , wherein said water in step a) is 10 times by weight of said dry powdered Spirulina. 
     
     
       44. The composition according to  claim 41 , wherein said step b) is conducted at a temperature of 80° C.-95° C. 
     
     
       45. The composition according to  claim 44 , wherein said step b) is conducted at a temperature of 90° C. 
     
     
       46. The composition according to  claim 41 , wherein step d) further comprises drying the filtrate. 
     
     
       47. The composition according to  claim 1 , wherein step d) further comprises drying the filtrate. 
     
     
       48. The composition according to  claim 6 , wherein step d) further comprises drying the filtrate. 
     
     
       49. The composition according to  claim 11 , wherein step d) further comprises drying the filtrate. 
     
     
       50. The composition according to  claim 16 , wherein step d) further comprises drying the filtrate. 
     
     
       51. The composition according to  claim 21 , wherein step d) further comprises drying the filtrate. 
     
     
       52. The composition according to  claim 26 , wherein step d) further comprises drying the filtrate. 
     
     
       53. The composition according to  claim 31 , wherein step d) further comprises drying the filtrate. 
     
     
       54. The process according to  claim 36 , wherein step d) further comprises drying the filtrate.

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