Alkaline, protease VapK suitable for laundry detergent, VapK gene, recombinant expression vector, and transformed microorganisms

Abstract

A novel alkaline protease VapK suitable for a laundry detergent is disclosed. The gene vapk coding for the protease VapK, the recombinant plasmids containing said gene, and the transformed V. metshnikovii KS1 (pSBCm) with said recombinant plasmid are also disclosed. In addition, a process for producing the protease VapK is disclosed.

1. An isolated alkaline protease gene comprising the nucleotide sequence of SEQ ID NO:2:
                 agcttctaat acgactcact atagggaaag ctttgctttc     40     ggttttttct gccgcttgac agatatttat gcgattcata      80     atggataaat aatcattata aatcgccatg atgtaaatca     120     agtagactaa aaaacgtaca gtttttttta cttaatagtc     160     tatcaatatc attaatttaa ccaataggta acaattcagt     200     aaataaaagc aaacacattc acccagctaa taatagcttt     240     attaataaac tataaaactg gcgaatggct gccgttgtaa     280     tgatccttga tgagtggtat ttgccactaa cattttacaa     320     ggataaaaaa atgttgaaga aaaatgtcaa ccgtacagta     360     ctggctgggt tattgttgcc aacttcaatc tcactggcaa     400     tagcatctca gcttaaggat caagaagtac cgagttttac     440     cccctctgtt gctgttgaaa atcatcaaac agaacaacgc     480     tattttgtta cctacgtgcc tggggcaacc agcggaccaa     520     tgcggatgag tcaaaacggc ttaacagaaa cagatttctc     560     tctgcaaaaa gccgccgata tattaagtac tcagcaagta     600     acggtcatca atcacctcga gtcattacat acttcagtgg     640     ttagagtgac gccaactcaa gccaagcaac tgctcgataa     680     tgctgatgtg gcgatgatcg aagtcgaccc aatacgctat     720     ttattcgatg ctgagattga gccttacgca caacagaccc     760     catacggaat ccgtatggta caagccgatc aactctctga     800     cgtttatgcg gctaatcgta aactttgcgt catcgactcg     840     ggatatcttc gcaaccatgt tgatctaccg agcgctggag     880     tcacaggcag cactttctct ggccatggtt catggttcac     920     tgatggcaat ggtcatggaa ctcacgttgc agggacaatt     960     gtcgcactgg ataataatgt cggagttgtt ggggttctac   1000     cgtctggctt agtcggccta cacaacgtaa aaatctttaa   1040     cgattccggt gtctggactc gcgcttcgga tttgattcaa   1080     gctatccaat cttgtcaaag tgcaggcagt catgtggtaa   1120     atatgagttt aggtggtagc caaggcagtg taaccgaaca   1160     aaagccaatg cgtaactttt accaacaagg gatgctctta   1200     gttgcagcag caggtaactc aggaaacagc ggcttctcat   1240     acccggcgtc ttatgatgca gtggtctcag ttgcggcggt   1280     taactcaagt ggtaatgtgg ctaacttctc acagttcaat   1320     tcacaagttg aactctcggc accaggagtt ggggtactat   1360     caaccggtaa taatggcggc tacttaagct atagcggaac   1400     ctcaatggct tcacctcacg ttgcaggtgt cgcagcgctg   1440     gtttggagtc actttccaca atgtcgacca gagcgaatcc   1480     gtcagtcact cagtcaaacg gctctcgatc gtggtgccgc   1520     aggtagagat aatttttacg gttgggggat agttcaagcg   1560     agacgtgcct ataactggtt atctcgcaat ggctgttaat   1600     ttctaatatt gagaatatga acagggtact gagtaccctg   1640     tttgttattt tccagagaca aatctaaccg tctaaattaa   1680     tttgtttaat caattcttct tgtacacggt ctgcctcaga   1720     taagggaatt tgacaggttc cagcggcatg atgaccgccg   1760     ccaccatatt tgagcattaa cgcaccaaca ttggtttgga   1800     actacgatct ttc   1813.                                                            
 
     
     
       2. A recombinant plasmid pSB1 containing the gene of  claim 1 . 
     
     
       3. A recombinant plasmid pSBCm containing the gene of  claim 1 . 
     
     
       4. A strain  Vibrio metschnikovii  KS1 transformed with the recombinant plasmid pSBCm of  claim 3 . 
     
     
       5. A process for producing a protease VapK suitable for a laundry detergent which comprises cultivation of the strain of  claim 4  under conditions permitting the expression of protease VapK and purifying the protease VapK from the culture.