Polynucleotide sequences which encode the zwa1 gene

Abstract

The invention relates to an isolated polynucleotide comprising a polynucleotide sequence chosen from the group consisting of a) polynucleotide which is identical to the extent of at least 70% to a polynucleotide which codes for a polypeptide which comprises the amino acid sequence of SEQ ID NO 2, b) polynucleotide which codes for a polypeptide which comprises an amino acid sequence which is identical to the extent of at least 70% to the amino acid sequence of SEQ ID NO 2 c) polynucleotide which is complementary to the polynucleotides of a) and b) and d) polynucleotide comprising at least 15 successive nucleotides of the polynucleotide sequence of a), b) or c), and processes for the fermentative preparation of L-amino acid with amplification of the zwa1 gene in the coryneform bacteria employed.

1. An isolated polynucleotide consisting of a polynucleotide sequence which encodes the protein of SEQ ID NO:2. 
     
     
       2. The isolated polynucleotide of  claim 1 , wherein said polynucleotide is isolated from the genus  Corynebacterium.    
     
     
       3. The isolated polynucleotide of  claim 1 , wherein said polynucleotide is isolated from the species  Corynebacterium glutamicum.    
     
     
       4. A vector comprising the isolated polynucleotide of any one of  claims 1  to  3 . 
     
     
       5. A An isolated host cell transformed with the isolated polynucleotide of any one of  claims 1  to  3 . 
     
     
       6. An isolated polynucleotide, comprising nucleotides 413 to 1009 of SEQ ID NO:1 or a degenerate variant thereof. 
     
     
       7. An isolated polynucleotide, comprising a full complement of the polynucleotide of  claim 6 . 
     
     
       8. An isolated polynucleotide comprising SEQ ID NO:1. 
     
     
       9. An isolated polynucleotide, comprising a full complement of the polynucleotide of  claim 8 . 
     
     
       10. An isolated polynucleotide consisting of SEQ ID NO:1 or a fragment thereof wherein said fragment encodes a protein consisting of the amino acid sequence of SEQ ID NO:2. 
     
     
       11. An isolated polynucleotide fragment of SEQ ID NO:1 consisting of at least 30 consecutive nucleotides of SEQ ID NO:1. 
     
     
       12. A vector comprising the isolated polynucleotide of any one of  claims 6  to  10 . 
     
     
       13. An isolated host cell transformed with the isolated polynucleotide of any one of  claims 6  to  10 . 
     
     
       14.  Escherichia coli  DSM 13115. 
     
     
       15. A coryneform bacterium serving as a host cell transformed with a vector comprising the isolated polynucleotide of  claim 1 . 
     
     
       16. A coryneform bacterium serving as a host cell transformed with a vector comprising the isolated polynucleotide of  claim 6 . 
     
     
       17. A coryneform bacterium serving as a host cell transformed with a vector comprising the isolated polynucleotide of SEQ ID NO:1. 
     
     
       18. An isolated polynucleotide, comprising a full complement of SEQ ID NO:1.