Pharmaceutical preparations useful for treating tumors and lesions of the skin and the mucous membranes and methods and kits using same

Abstract

A pharmaceutical preparation useful for treating a skin or mucous membrane lesion is provided. The pharmaceutical preparation including, as active ingredients, a therapeutically effective amount of trichloroacetic acid and hydrochloric acid, or trichloroacetic acid and formic acid or all three of these acids and optionally a crosslinking/fixating/preserving agent. Additional preparations are also described.

1. A method of removing and collecting a skin or mucous membrane lesion from a patient while preserving the morphology of the tissue thereof, the method comprising:
 (a) applying to the skin or mucous membrane lesion of said patient at least one preparation for mummification of a tissue, so as to obtain, following a mummification period, a mummified skin or mucous membrane lesion that dries and separates from a base tissue thereof and preserves the morphology of the tissue thereof; and 
 (b) collecting said mummified skin or mucous membrane lesion that has separated from the base tissue; 
 wherein said preparation for mummification of a tissue comprises, as active ingredients, an effective amount of a composition comprising trichloroacetic acid and one or both of hydrochloric acid and formic acid. 
 
     
     
       2. The method of  claim 1 , wherein said preparation for mummification of a tissue includes, as active ingredients, an effective amount of a composition comprising trichloroacetic acid, hydrochloric acid and formic acid. 
     
     
       3. The method of  claim 1 , wherein said trichloroacetic acid and one or both of hydrochloric acid and formic acid are present in said composition in relative concentrations of 5 to 97% (w/w) trichloroacetic acid, 3 to 38% (w/w) hydrochloric acid, if present, 3 to 85% (w/w) formic acid, if present. 
     
     
       4. The method of  claim 1 , wherein said preparation for mummification of a tissue further comprises at least one crosslinking/fixating/preserving agent. 
     
     
       5. The method of  claim 4 , wherein said at least one crosslinking/fixating/preserving agent is selected from group consisting of formaldehyde, paraformaldehyde, glutaraldehyde, glyoxal, carbodi-imide, a formaldehyde donor, sodium hydroxymethyl glycinate, diazolidinyl urea, imidazolidinyl urea, dimethylol-5,5-dimethylhydantoin, dimethylol urea, 2-bromo-2-nitropropane 1,3-diol, quaternium-15, parabens, 5-chloro-2methylisothiazolin-3-one, 1,2-dibromo-2,4-dicyanobutane, ethanol and other alcohols and polyol. 
     
     
       6. The method of  claim 1 , wherein said preparation for mummification of a tissue includes about 48% (w/w) trichloroacetic acid, about 8% (w/w) hydrochloric acid and about 3% (w/w) formic acid. 
     
     
       7. The method of  claim 6 , wherein said preparation for mummification of a tissue further includes 4% (w/w) formaldehyde. 
     
     
       8. The method of  claim 1 , wherein said preparation for mummification of a tissue further comprises at least one agent selected from the group consisting of monochloroacetic acid, glycolic acid, acetic acid, azelaic acid, phosphoric acid, thioglycolic acid, salicylic acid and their salts and phenol. 
     
     
       9. The method of  claim 8 , wherein said preparation for mummification of a tissue further comprises at least one crosslinking/fixating/preserving agent. 
     
     
       10. The method of  claim 9 , wherein said at least one crosslinking/fixating/preserving agent is selected from the group consisting of formaldehyde, paraformaldehyde, glutaraldehyde, glyoxal, carbodi-imide, a formaldehyde donor, sodium hydroxymethyl glycinate, diazolidinyl urea, imidazolidinyl urea, dimethylol-5,5-dimethylhydantoin, dimethylol urea, 2-bromo-2-nitropropane 1,3-diol, quaternium-15, parabens, 5-chloro-2methylisothiazolin-3-one, 1,2-dibromo-2,4-dicyanobutane, ethanol and other alcohols and polyol. 
     
     
       11. The method of  claim 8 , wherein said preparation for mummification of a tissue further comprises at least one additional agent comprising a nonoxidizing organic or inorganic acid. 
     
     
       12. The method of  claim 5 , wherein a concentration of said formaldehyde is selected from a range of 1% to 10% (w/w). 
     
     
       13. The method of  claim 10 , wherein a concentration of said formaldehyde is selected from a range of 1% to 10% (w/w). 
     
     
       14. The method of  claim 12 , wherein said concentration of formaldehyde is about 4% (w/w). 
     
     
       15. The method of  claim 13 , wherein said concentration of formaldehyde is about 4% (w/w). 
     
     
       16. The method of  claim 1 , further including the steps of:
 (c) rehydrating said mummified skin or mucous membrane lesion collected in said step (b); and 
 (d) histopathologically examining said mummified skin or mucous membrane lesion. 
 
     
     
       17. The method of  claim 16 , wherein said preparation for mummification of a tissue includes, as active ingredients, an effective amount of a composition comprising trichloroacetic acid, hydrochloric acid and formic acid. 
     
     
       18. The method of  claim 16 , wherein said trichloroacetic acid and one or both of hydrochloric acid and formic acid are present in said composition in relative concentrations of 5to 97% (w/w) trichloroacetic acid, 3 to 38% (w/w) hydrochloric acid, if present, and 3 to 85% (w/w) formic acid, if present. 
     
     
       19. The method of  claim 16 , wherein said preparation for mummification of a tissue further comprises at least one crosslinking/fixating/preserving agent. 
     
     
       20. The method of  claim 19 , wherein said at least one crosslinking/fixating/preserving agent is selected from group consisting of formaldehyde, paraformaldehyde, glutaraldehyde, glyoxal, carbodi-imide, a formaldehyde donor, sodium hydroxymethyl glycinate, diazolidinyl urea, imidazolidinyl urea, dimethylol-5,5-dimethylhydantoin, dimethylol urea, 2-bromo-2-nitropropane 1,3-diol, quaternium-15, parabens, 5-chloro-2methylisothiazolin-30-one, 1,2-dibromo-2,4-dicyanobutane, ethanol and other alcohols and polyol. 
     
     
       21. The method of  claim 16 , wherein said preparation for mummification of a tissue includes about 48% (w/w) trichloroacetic acid, about 8% (w/w) hydrochloric acid and about 3% (w/w) formic acid. 
     
     
       22. The method of  claim 21 , wherein said preparation for mummification of a tissue further includes 4% (w/w) formaldehyde. 
     
     
       23. The method of  claim 16 , further comprising staining said mummified skin or mucous membrane lesion prior to step (d). 
     
     
       24. The method of  claim 16 , wherein said preparation for mummification of a tissue further comprises at least one agent selected from the group consisting of monochloroacetic acid, glycolic acid, acetic acid, azelaic acid, phosphoric acid, thioglycolic acid, salicylic acid and their salts and phenol. 
     
     
       25. The method of  claim 24 , wherein said preparation for mummification of a tissue further comprises at least one crosslinking/fixating/preserving agent. 
     
     
       26. The method of  claim 25 , wherein said at least one crosslinking/fixating/preserving agent is selected from the group consisting of formaldehyde, paraformaldehyde, glutaraldehyde, glyoxal, carbodi-imide, a formaldehyde donor, sodium hydroxymethyl glycinate, diazolidinyl urea, imidazolidinyl urea, dimethylol-5,5-dimethylhydantoin, dimethylol urea, 2-bromo-2-nitropropane 1,3-diol, quaternium-15, parabens, 5-chloro-2methylisothiazolin-3-one, 1,2-dibromo-2,4-dicyanobutane, ethanol and other alcohols and polyol. 
     
     
       27. The method of  claim 24 , wherein said preparation for mummification of a tissue further comprises at least one additional agent comprising nonoxidizing organic or inorganic acid. 
     
     
       28. The method of  claim 20 , wherein a concentration of said formaldehyde is selected from a range of 1% to 10% (w/w). 
     
     
       29. The method of  claim 26 , wherein a concentration of said formaldehyde is selected from a range of 1% to 10% (w/w). 
     
     
       30. The method of  claim 28 , wherein said concentration of formaldehyde is about 4% (w/w). 
     
     
       31. The method of  claim 29 , wherein said concentration of formaldehyde is about 4% (w/w).