US7189694B2ExpiredUtilityA1

Inhibitors of autophosphorylation protein kinases

61
Assignee: UNIV FLORIDAPriority: Apr 18, 2003Filed: Apr 19, 2004Granted: Mar 13, 2007
Est. expiryApr 18, 2023(expired)· nominal 20-yr term from priority
Inventors:Howard M. JohnsonPrem S. SubramaniamMustafa G. MujtabaLawrence Flowers
A61P 35/00A61P 37/00A61P 9/00C07K 14/71A61K 38/00C07K 14/4703A61P 29/00
61
PatentIndex Score
3
Cited by
48
References
50
Claims

Abstract

The subject invention concerns peptide molecules that specifically inhibit the enzymatic function of tyrosine kinases, including the JAK and EGF receptor (EGFR) family of kinases, to autophosphorylate, i.e., to transfer a phosphate group from ATP to an amino acid in the kinase. Phosphorylation of proteins is the most fundamental method for signal transduction among proteins in a cell. Inhibition of tyrosine kinase autophosphorylation activities inhibits the enzyme's signaling and shuts down the functioning pathways originating from the enzyme. The JAK2 and EGFR tyrosine kinases are involved in both inflammatory disorders and cancer. In these disorders, the tyrosine kinases can often be activated in an uncontrolled fashion. The subject application also concerns antibodies that bind to a tyrosine kinase autophosphorylation site. The subject invention also concerns pharmaceutically acceptable formulations of the subject peptides and antibodies, and methods for treating inflammatory and oncological disorders by inhibiting tyrosine kinase signaling in these situations by administering a peptide or antibody of the present invention.

Claims

exact text as granted — not AI-modified
1. A peptide comprising the amino acid sequence shown in SEQ ID NO. 1, SEQ ID NO. 3, SEQ ID NO. 4, SEQ ID NO. 5, SEQ ID NO. 6. SEQ ID NO. 7, SEQ ID NO. 8, SEQ ID NO. 9, SEQ ID NO. 10, SEQ ID NO. 11, SEQ ID NO. 12, SEQ ID NO. 13, SEQ ID NO. 14, SEQ ID NO. 15, SEQ ID NO. 16, SEQ ID NO. 17, SEQ ID NO. 18, SEQ ID NO. 19, SEQ ID NO. 20, SEQ ID NO. 21, SEQ ID NO. 22, SEQ ID NO. 23, SEQ ID NO. 24, SEQ ID NO. 25, SEQ ID NO. 26, SEQ ID NO. 27, SEQ ID NO. 28, SEQ ID NO. 29, SEQ ID NO. 30, SEQ ID NO. 31, SEQ ID NO. 32, SEQ ID NO. 33, SEQ ID NO. 34, SEQ ID NO. 35, or SEQ ID NO. 36, wherein said peptide can bind to an autophosphorylation site of a protein kinase and thereby inhibit autophosphorylation of said protein kinase. 
     
     
       2. The peptide according to  claim 1 , wherein said protein kinase is a tyrosine kinase. 
     
     
       3. The peptide according to  claim 2 , wherein said tyrosine kinase is a JAK2 polypeptide. 
     
     
       4. The peptide according to  claim 1 , wherein said peptide consists of between about 5 to about 45 amino acids. 
     
     
       5. The peptide according to  claim 1 , wherein said peptide binds to an autophosphorylation site of said protein kinase and said autophosphorylation site has the amino acid sequence shown in SEQ ID NO. 2. 
     
     
       6. The peptide according to  claim 1 , wherein said peptide consists of the amino acid sequence shown in SEQ ID NO. 1, SEQ ID NO. 3, SEQ ID NO. 4, SEQ ID NO. 5, SEQ ID NO. 6. SEQ ID NO. 7, SEQ ID NO. 8, SEQ ID NO. 9, SEQ ID NO. 10, SEQ ID NO. 11, SEQ ID NO. 12, SEQ ID NO. 13, SEQ ID NO. 14, SEQ ID NO. 15, SEQ ID NO. 16, SEQ ID NO. 17, SEQ ID NO. 18, SEQ ID NO. 19, SEQ ID NO. 20, SEQ ID NO. 21, SEQ ID NO. 22, SEQ ID NO. 23, SEQ ID NO. 24, SEQ ID NO. 25, SEQ ID NO. 26, SEQ ID NO. 27, SEQ ID NO. 28, SEQ ID NO. 29, SEQ ID NO. 30, SEQ ID NO. 31, SEQ ID NO. 32, SEQ ID NO. 33, SEQ ID NO. 34, SEQ ID NO. 35, or SEQ ID NO. 36. 
     
     
       7. A composition comprising a peptide, wherein said peptide comprises the amino acid sequence shown in SEQ ID NO. 1, SEQ ID NO. 3, SEQ ID NO. 4, SEQ ID NO. 5, SEQ ID NO. 6. SEQ ID NO. 7, SEQ ID NO. 8, SEQ ID NO. 9, SEQ ID NO. 10, SEQ ID NO. 11, SEQ ID NO. 12, SEQ ID NO. 13, SEQ ID NO. 14, SEQ ID NO. 15, SEQ ID NO. 16, SEQ ID NO. 17, SEQ ID NO. 18, SEQ ID NO. 19, SEQ ID NO. 20, SEQ ID NO. 21, SEQ ID NO. 22, SEQ ID NO. 23, SEQ ID NO. 24, SEQ ID NO. 25, SEQ ID NO. 26, SEQ ID NO. 27, SEQ ID NO. 28, SEQ ID NO. 29, SEQ ID NO. 30, SEQ ID NO. 31, SEQ ID NO. 32, SEQ ID NO. 33, SEQ ID NO. 34, SEQ ID NO. 35, or SEQ ID NO. 36, and wherein said peptide can bind to an autophosphorylation site of a protein kinase and thereby inhibit autophosphorylation of said protein kinase. 
     
     
       8. The composition according to  claim 7 , wherein said protein kinase is a tyrosine kinase. 
     
     
       9. The composition according to  claim 8 , wherein said tyrosine kinase is a JAK2 polypeptide. 
     
     
       10. The composition according to  claim 7 , wherein said peptide consists of between about 5 to about 45 amino acids. 
     
     
       11. The composition according to  claim 7 , wherein said composition comprises a pharmaceutically-acceptable carrier or diluent. 
     
     
       12. The composition according to  claim 7 , wherein said peptide binds to an autophosphorylation site of said protein kinase and said autophosphorylation site has the amino acid sequence shown in SEQ ID NO. 2. 
     
     
       13. The composition according to  claim 7 , wherein said peptide consists of the amino acid sequence shown in SEQ ID NO. 1, SEQ ID NO. 3, SEQ ID NO. 4, SEQ ID NO. 5, SEQ ID NO. 6. SEQ ID NO. 7, SEQ ID NO. 8, SEQ ID NO. 9, SEQ ID NO. 10, SEQ ID NO. 11, SEQ ID NO. 12, SEQ ID NO. 13, SEQ ID NO. 14, SEQ ID NO. 15, SEQ ID NO. 16, SEQ ID NO. 17, SEQ ID NO. 18, SEQ ID NO. 19, SEQ ID NO. 20, SEQ ID NO. 21, SEQ ID NO. 22, SEQ ID NO. 23, SEQ ID NO. 24, SEQ ID NO. 25, SEQ ID NO. 26, SEQ ID NO. 27, SEQ ID NO. 28, SEQ ID NO. 29, SEQ ID NO. 30, SEQ ID NO. 31, SEQ ID NO. 32, SEQ ID NO. 33, SEQ ID NO. 34, SEQ ID NO. 35, or SEQ ID NO. 36. 
     
     
       14. An isolated peptide that consists of the sequence shown in SEQ ID NO. 1, SEQ ID NO. 3, SEQ ID NO. 4, SEQ ID NO. 5, SEQ ID NO. 6. SEQ ID NO. 7, SEQ ID NO. 8, SEQ ID NO. 9, SEQ ID NO. 10, SEQ ID NO. 11, SEQ ID NO. 12, SEQ ID NO. 13, SEQ ID NO. 14, SEQ ID NO. 15, SEQ ID NO. 16, SEQ ID NO. 17, SEQ ID NO. 18, SEQ ID NO. 19, SEQ ID NO. 20, SEQ ID NO. 21, SEQ ID NO. 22, SEQ ID NO. 23, SEQ ID NO. 24, SEQ ID NO. 25, SEQ ID NO. 26, SEQ ID NO. 27, SEQ ID NO. 28, SEQ ID NO. 29, SEQ ID NO. 30, SEQ ID NO. 31, SEQ ID NO. 32, SEQ ID NO. 33, SEQ ID NO. 34, SEQ ID NO. 35, or SEQ ID NO. 36, wherein said peptide can bind to an autophosphorylation site of a protein kinase and thereby inhibit autophosphorylation of said protein kinase. 
     
     
       15. The isolated peptide according to  claim 14 , wherein said protein kinase is a tyrosine kinase. 
     
     
       16. The isolated peptide according to  claim 15 , wherein said tyrosine kinase is a JAK2 polypeptide. 
     
     
       17. The isolated peptide according to  claim 14 , wherein said peptide binds to an autophosphorylation site of said protein kinase and said autophosphorylation site has the amino acid sequence shown in SEQ ID NO. 2. 
     
     
       18. A method for treating or preventing an autoimmune, inflammatory, cardiovascular, or oncological disorder in a human or animal, said method comprising administering to the human or animal an effective amount of:
 a) a peptide comprising the amino acid sequence shown in SEQ ID NO. 1, SEQ ID NO. 3, SEQ ID NO. 4, SEQ ID NO. 5, SEQ ID NO. 6. SEQ ID NO. 7, SEQ ID NO. 8, SEQ ID NO. 9, SEQ ID NO. 10, SEQ ID NO. 11, SEQ ID NO. 12, SEQ ID NO. 13, SEQ ID NO. 14, SEQ ID NO. 15, SEQ ID NO. 16, SEQ ID NO. 17, SEQ ID NO. 18, SEQ ID NO. 19, SEQ ID NO. 20, SEQ ID NO. 21, SEQ ID NO. 22, SEQ ID NO. 23, SEQ ID NO. 24, SEQ ID NO. 25, SEQ ID NO. 26, SEQ ID NO. 27, SEQ ID NO. 28, SEQ ID NO. 29, SEQ ID NO. 30, SEQ ID NO. 31, SEQ ID NO. 32, SEQ ID NO. 33, SEQ ID NO. 34, SEQ ID NO. 35, or SEQ ID NO. 36, wherein said peptide can bind to an autophosphorylation site of a protein kinase and thereby inhibit autophosphorylation of said protein kinase; 
 b) a composition comprising a peptide, wherein said peptide comprises the amino acid sequence shown in SEQ ID NO. 1, SEQ ID NO. 3, SEQ ID NO. 4, SEQ ID NO. 5, SEQ ID NO. 6. SEQ ID NO. 7, SEQ ID NO. 8, SEQ ID NO. 9, SEQ ID NO. 10, SEQ ID NO. 11, SEQ ID NO. 12, SEQ ID NO. 13, SEQ ID NO. 14, SEQ ID NO. 15, SEQ ID NO. 16, SEQ ID NO. 17, SEQ ID NO. 18, SEQ ID NO. 19, SEQ ID NO. 20, SEQ ID NO. 21, SEQ ID NO. 22, SEQ ID NO. 23, SEQ ID NO. 24, SEQ ID NO. 25, SEQ ID NO. 26, SEQ ID NO. 27, SEQ ID NO. 28, SEQ ID NO. 29, SEQ ID NO. 30, SEQ ID NO. 31, SEQ ID NO. 32, SEQ ID NO. 33, SEQ ID NO. 34, SEQ ID NO. 35, or SEQ ID NO. 36, and wherein said peptide can bind to an autophosphorylation site of a protein kinase and thereby inhibit autophosphorylation of said protein kinase; or 
 c) an isolated peptide that consists of the sequence shown in SEQ ID NO. 1, SEQ ID NO. 3, SEQ ID NO. 4, SEQ ID NO. 5, SEQ ID NO. 6. SEQ ID NO. 7, SEQ ID NO. 8, SEQ ID NO. 9, SEQ ID NO. 10, SEQ ID NO. 11, SEQ ID NO. 12, SEQ ID NO. 13, SEQ ID NO. 14, SEQ ID NO. 15, SEQ ID NO. 16, SEQ ID NO. 17, SEQ ID NO. 18, SEQ ID NO. 19, SEQ ID NO. 20, SEQ ID NO. 21, SEQ ID NO. 22, SEQ ID NO. 23, SEQ ID NO. 24, SEQ ID NO. 25, SEQ ID NO. 26, SEQ ID NO. 27, SEQ ID NO. 28, SEQ ID NO. 29, SEQ ID NO. 30, SEQ ID NO. 31, SEQ ID NO. 32, SEQ ID NO. 33, SEQ ID NO. 34, SEQ ID NO. 35, or SEQ ID NO. 36, wherein said peptide can bind to an autophosphorylation site of a protein kinase and thereby inhibit autophosphorylation of said protein kinase. 
 
     
     
       19. The method according to  claim 18 , wherein said inflammatory disorder is selected from the group consisting of arthritis, multiple sclerosis, lupus, Crohn's disease, diabetes, graft rejection, neurological disease, and an inflammatory connective tissue disease. 
     
     
       20. The method according to  claim 18 , wherein said oncological disorder is selected from the group consisting of cancer and/or tumors of the breast, kidney, mouth, larynx, esophagus, stomach, colon, ovary, lung, bladder, skin, muscle, pancreas, prostate, blood cells, and brain. 
     
     
       21. The method according to  claim 18 , wherein said oncological disorder is a leukemia. 
     
     
       22. A method for inhibiting autophosphorylation of a protein kinase, said method comprising contacting said protein kinase with:
 a) a peptide comprising the amino acid sequence shown in SEQ ID NO. 1, SEQ ID NO. 3, SEQ ID NO. 4, SEQ ID NO. 5, SEQ ID NO. 6. SEQ ID NO. 7, SEQ ID NO. 8, SEQ ID NO. 9, SEQ ID NO. 10, SEQ ID NO. 11, SEQ ID NO. 12, SEQ ID NO. 13, SEQ ID NO. 14, SEQ ID NO. 15, SEQ ID NO. 16, SEQ ID NO. 17, SEQ ID NO. 18, SEQ ID NO. 19, SEQ ID NO. 20, SEQ ID NO. 21, SEQ ID NO. 22, SEQ ID NO. 23, SEQ ID NO. 24, SEQ ID NO. 25, SEQ ID NO. 26, SEQ ID NO. 27, SEQ ID NO. 28, SEQ ID NO. 29, SEQ ID NO. 30, SEQ ID NO. 31, SEQ ID NO. 32, SEQ ID NO. 33, SEQ ID NO. 34, SEQ ID NO. 35, or SEQ ID NO. 36, wherein said peptide can bind to an autophosphorylation site of said protein kinase and thereby inhibit autophosphorylation of said protein kinase; 
 b) a composition comprising a peptide, wherein said peptide comprises the amino acid sequence shown in SEQ ID NO. 1, SEQ ID NO. 3, SEQ ID NO. 4, SEQ ID NO. 5, SEQ ID NO. 6. SEQ ID NO. 7, SEQ ID NO. 8, SEQ ID NO. 9, SEQ ID NO. 10, SEQ ID NO. 11, SEQ ID NO. 12, SEQ ID NO. 13, SEQ ID NO. 14, SEQ ID NO. 15, SEQ ID NO. 16, SEQ ID NO. 17, SEQ ID NO. 18, SEQ ID NO. 19, SEQ ID NO. 20, SEQ ID NO. 21, SEQ ID NO. 22, SEQ ID NO. 23, SEQ ID NO. 24, SEQ ID NO. 25, SEQ ID NO. 26, SEQ ID NO. 27, SEQ ID NO. 28, SEQ ID NO. 29, SEQ ID NO. 30, SEQ ID NO. 31, SEQ ID NO. 32, SEQ ID NO. 33, SEQ ID NO. 34, SEQ ID NO. 35, or SEQ ID NO. 36, and wherein said peptide can bind to an autophosphorylation site of said protein kinase and thereby inhibit autophosphorylation of said protein kinase; or 
 c) an isolated peptide that consists of the sequence shown in SEQ ID NO. 1, SEQ ID NO. 3, SEQ ID NO. 4, SEQ ID NO. 5, SEQ ID NO. 6. SEQ ID NO. 7, SEQ ID NO. 8, SEQ ID NO. 9, SEQ ID NO. 10, SEQ ID NO. 11, SEQ ID NO. 12, SEQ ID NO. 13, SEQ ID NO. 14, SEQ ID NO. 15, SEQ ID NO. 16, SEQ ID NO. 17, SEQ ID NO. 18, SEQ ID NO. 19, SEQ ID NO. 20, SEQ ID NO. 21, SEQ ID NO. 22, SEQ ID NO. 23, SEQ ID NO. 24, SEQ ID NO. 25, SEQ ID NO. 26, SEQ ID NO. 27, SEQ ID NO. 28, SEQ ID NO. 29, SEQ ID NO. 30, SEQ ID NO. 31, SEQ ID NO. 32, SEQ ID NO. 33, SEQ ID NO. 34, SEQ ID NO. 35, or SEQ ID NO. 36, wherein said peptide can bind to an autophosphorylation site of said protein kinase and thereby inhibit autophosphorylation of said protein kinase. 
 
     
     
       23. The method according to  claim 22 , wherein said protein kinase is a tyrosine kinase. 
     
     
       24. The method according to  claim 23 , wherein said tyrosine kinase is a JAK2 polypeptide. 
     
     
       25. The method according to  claim 23 , wherein said tyrosine kinase is an EGFR polypeptide. 
     
     
       26. The method according to  claim 23 , wherein said tyrosine kinase is erbB-1, erbB-2, erbB-3, or erbB-4. 
     
     
       27. The method according to  claim 22 , wherein said autophosphorylation site of said protein kinase comprises the amino acid sequence shown in SEQ ID NO. 2. 
     
     
       28. A kit comprising in one or more containers:
 a) a peptide comprising the amino acid sequence shown in SEQ ID NO. 1, SEQ ID NO. 3, SEQ ID NO. 4, SEQ ID NO. 5, SEQ ID NO. 6. SEQ ID NO. 7, SEQ ID NO. 8, SEQ ID NO. 9, SEQ ID NO. 10, SEQ ID NO. 11, SEQ ID NO. 12, SEQ ID NO. 13, SEQ ID NO. 14, SEQ ID NO. 15, SEQ ID NO. 16, SEQ ID NO. 17, SEQ ID NO. 18, SEQ ID NO. 19, SEQ ID NO. 20, SEQ ID NO. 21, SEQ ID NO. 22, SEQ ID NO. 23, SEQ ID NO. 24, SEQ ID NO. 25, SEQ ID NO. 26, SEQ ID NO. 27, SEQ ID NO. 28, SEQ ID NO. 29, SEQ ID NO. 30, SEQ ID NO. 31, SEQ ID NO. 32, SEQ ID NO. 33, SEQ ID NO. 34, SEQ ID NO. 35, or SEQ ID NO. 36, wherein said peptide can bind to an autophosphorylation site of a protein kinase and thereby inhibit autophosphorylation of said protein kinase; 
 b) a composition comprising a peptide, wherein said peptide comprises the amino acid sequence shown in SEQ ID NO. 1, SEQ ID NO. 3, SEQ ID NO. 4, SEQ ID NO. 5, SEQ ID NO. 6 SEQ ID NO. 7, SEQ ID NO. 8, SEQ ID NO. 9, SEQ ID NO. 10, SEQ ID NO. 11, SEQ ID NO. 12, SEQ ID NO. 13, SEQ ID NO. 14, SEQ ID NO. 15, SEQ ID NO. 16, SEQ ID NO. 17, SEQ ID NO. 18, SEQ ID NO. 19, SEQ ID NO. 20, SEQ ID NO. 21, SEQ ID NO. 22, SEQ ID NO. 23, SEQ ID NO. 24, SEQ ID NO. 25, SEQ ID NO. 26, SEQ ID NO. 27, SEQ ID NO. 28, SEQ ID NO. 29, SEQ ID NO. 30, SEQ ID NO. 31, SEQ ID NO. 32, SEQ ID NO. 33, SEQ ID NO. 34, SEQ ID NO. 35, or SEQ ID NO. 36, and wherein said peptide can bind to an autophosphorylation site of a protein kinase and thereby inhibit autophosphorylation of said protein kinase; or 
 c) an isolated peptide that consists of the sequence shown in SEQ ID NO. 1, SEQ ID NO. 3, SEQ ID NO. 4, SEQ ID NO. 5, SEQ ID NO. 6, SEQ ID NO. 7, SEQ ID NO. 8, SEQ ID NO. 9, SEQ ID NO. 10, SEQ ID NO. 11, SEQ ID NO. 12, SEQ ID NO. 13, SEQ ID NO. 14, SEQ ID NO. 15, SEQ ID NO. 16, SEQ ID NO. 17, SEQ ID NO. 18, SEQ ID NO. 19, SEQ ID NO. 20, SEQ ID NO. 21, SEQ ID NO. 22, SEQ ID NO. 23, SEQ ID NO. 24, SEQ ID NO. 25, SEQ ID NO. 26, SEQ ID NO. 27, SEQ ID NO. 28, SEQ ID NO. 29, SEQ ID NO. 30, SEQ ID NO. 31, SEQ ID NO. 32, SEQ ID NO. 33, SEQ ID NO. 34, SEQ ID NO. 35, or SEQ ID NO. 36, wherein said peptide can bind to an autophosphorylation site of a protein kinase and thereby inhibit autophosphorylation of said protein kinase. 
 
     
     
       29. A method for inhibiting IFN-γ mediated activity of a cell, said method comprising contacting said cell with an effective amount of:
 a) a peptide comprising the amino acid sequence shown in SEQ ID NO. 1, SEQ ID NO. 3, SEQ ID NO. 4, SEQ ID NO. 5, SEQ ID NO. 6. SEQ ID NO. 7, SEQ ID NO. 8, SEQ ID NO. 9, SEQ ID NO. 10, SEQ ID NO. 11, SEQ ID NO. 12, SEQ ID NO. 13, SEQ ID NO. 14, SEQ ID NO. 15, SEQ ID NO. 16, SEQ ID NO. 17, SEQ ID NO. 18, SEQ ID NO. 19, SEQ ID NO. 20, SEQ ID NO. 21, SEQ ID NO. 22, SEQ ID NO. 23, SEQ ID NO. 24, SEQ ID NO. 25, SEQ ID NO. 26, SEQ ID NO. 27, SEQ ID NO. 28, SEQ ID NO. 29, SEQ ID NO. 30, SEQ ID NO. 31, SEQ ID NO. 32, SEQ ID NO. 33, SEQ ID NO. 34, SEQ ID NO. 35, or SEQ ID NO. 36, wherein said peptide can bind to an autophosphorylation site of a protein kinase and thereby inhibit autophosphorylation of said protein kinase; 
 b) a composition comprising a peptide, wherein said peptide comprises the amino acid sequence shown in SEQ ID NO. 1, SEQ ID NO. 3, SEQ ID NO. 4, SEQ ID NO. 5, SEQ ID NO. 6. SEQ ID NO. 7, SEQ ID NO. 8, SEQ ID NO. 9, SEQ ID NO. 10, SEQ ID NO. 11, SEQ ID NO. 12, SEQ ID NO. 13, SEQ ID NO. 14, SEQ ID NO. 15, SEQ ID NO. 16, SEQ ID NO. 17, SEQ ID NO. 18, SEQ ID NO. 19, SEQ ID NO. 20, SEQ ID NO. 21, SEQ ID NO. 22, SEQ ID NO. 23, SEQ ID NO. 24, SEQ ID NO. 25, SEQ ID NO. 26, SEQ ID NO. 27, SEQ ID NO. 28, SEQ ID NO. 29, SEQ ID NO. 30, SEQ ID NO. 31, SEQ ID NO. 32, SEQ ID NO. 33, SEQ ID NO. 34, SEQ ID NO. 35, or SEQ ID NO. 36, and wherein said peptide can bind to an autophosphorylation site of a protein kinase and thereby inhibit autophosphorylation of said protein kinase; or 
 c) an isolated peptide that consists of the sequence shown in SEQ ID NO. 1, SEQ ID NO. 3, SEQ ID NO. 4, SEQ ID NO. 5, SEQ ID NO. 6. SEQ ID NO. 7, SEQ ID NO. 8, SEQ ID NO. 9, SEQ ID NO. 10, SEQ ID NO. 11, SEQ ID NO. 12, SEQ ID NO. 13, SEQ ID NO. 14, SEQ ID NO. 15, SEQ ID NO. 16, SEQ ID NO. 17, SEQ ID NO. 18, SEQ ID NO. 19, SEQ ID NO. 20, SEQ ID NO. 21, SEQ ID NO. 22, SEQ ID NO. 23, SEQ ID NO. 24, SEQ ID NO. 25, SEQ ID NO. 26, SEQ ID NO. 27, SEQ ID NO. 28, SEQ ID NO. 29, SEQ ID NO. 30, SEQ ID NO. 31, SEQ ID NO. 32, SEQ ID NO. 33, SEQ ID NO. 34, SEQ ID NO. 35, or SEQ ID NO. 36, wherein said peptide can bind to an autophosphorylation site of a protein kinase and thereby inhibit autophosphorylation of said protein kinase. 
 
     
     
       30. The method according to  claim 29 , wherein said IFN-γ mediated activity is antiviral activity. 
     
     
       31. The method according to  claim 30 , wherein said IFN-γ mediated activity is upregulation of MHC class I molecules on cells. 
     
     
       32. The peptide according to  claim 1 , wherein said peptide comprises a chemical group that decreases an immunogenic response to said peptide or that extends the half-life of said peptide in vivo. 
     
     
       33. The peptide according to  claim 32 , wherein said chemical group is polyethylene glycol (PEG). 
     
     
       34. The peptide according to  claim 1 , wherein said peptide further comprises an amino acid sequence that allows said peptide to be translocated across a biological membrane. 
     
     
       35. The peptide according to  claim 1 , wherein said peptide consists of between about 5 to about 35 amino acids. 
     
     
       36. The composition according to  claim 7 , wherein said peptide comprises a chemical group that decreases an immunogenic response to said peptide or that extends the half-life of said peptide in vivo. 
     
     
       37. The composition according to  claim 36 , wherein said chemical group is polyethylene glycol (PEG). 
     
     
       38. The composition according to  claim 7 , wherein said peptide further comprises an amino acid sequence that allows said peptide to be translocated across a biological membrane. 
     
     
       39. The composition according to  claim 7 , wherein said peptide consists of between about 5 to about 35 amino acids. 
     
     
       40. The isolated peptide according to  claim 14 , wherein said peptide comprises a chemical group that decreases an immunogenic response to said peptide or that extends the half-life of said peptide in vivo. 
     
     
       41. The isolated peptide according to  claim 40 , wherein said chemical group is polyethylene glycol (PEG). 
     
     
       42. The method according to  claim 18 , wherein said peptide comprises a chemical group that decreases an immunogenic response to said peptide or that extends the half-life of said peptide in vivo. 
     
     
       43. The method according to  claim 42 , wherein said chemical group is polyethylene glycol (PEG). 
     
     
       44. The method according to  claim 22 , wherein said peptide comprises a chemical group that decreases an immunogenic response to said peptide or that extends the half-life of said peptide in vivo. 
     
     
       45. The method according to  claim 44 , wherein said chemical group is polyethylene glycol (PEG). 
     
     
       46. The kit according to  claim 28 , wherein said peptide comprises a chemical group that decreases an immunogenic response to said peptide or that extends the half-life of said peptide in vivo. 
     
     
       47. The kit according to  claim 46 , wherein said chemical group is polyethylene glycol (PEG). 
     
     
       48. The method according to  claim 29 , wherein said peptide comprises a chemical group that decreases an immunogenic response to said peptide or that extends the half-life of said peptide in vivo. 
     
     
       49. The method according to  claim 48 , wherein said chemical group is polyethylene glycol (PEG). 
     
     
       50. The method according to  claim 18 , wherein said disorder is an oncological disorder and said method further comprises administering one or more of an antitumor substance, radiation therapy, and surgical treatment.

Join the waitlist — get patent alerts

Track US7189694B2 — get alerts on status changes and closely related new filings.

We store only your email — no account needed. See our privacy policy.