Parvovirus vectors and their use

Abstract

This invention relates to a parvovirus vector having a parvovirus DNA excisable from the vector DNA in a parvovirus-permissive cell, the parvovirus DNA having a left terminus which comprises a parvovirus minimal origin of replication, and a system comprising the parvovirus vector. Furthermore, this invention concerns a method of producing the parvovirus vector, parvoviral particles as well as their use.

1. A parvovirus vector comprising a parvovirus DNA having a left terminus which comprises a parvovirus minimal origin of replication comprising CTWWTCA, wherein W is any nucleotide, and the parvovirus DNA is excisable from the parvovirus vector in a parvovirus-permissive cell, wherein the parvovirus DNA region coding for capsid proteins is partially or fully replaced by an exogeneous DNA coding for a chemotactic polypeptide. 
     
     
       2. The parvovirus vector according to  claim 1 , wherein the parvovirus DNA originates from a mammalian parvovirus. 
     
     
       3. The parvovirus vector according to  claim 1 , wherein the parvovirus DNA is a rodent parvovirus. 
     
     
       4. The parvovirus vector according to  claim 3 , wherein the rodent parvovirus is MVM or H-1. 
     
     
       5. The parvovirus vector according to  claim 1 , wherein the chemotactic polypeptide is MCP-1. 
     
     
       6. The parvovirus vector according  claim 1 , wherein the parvovirus vector is present as a parvoviral particle. 
     
     
       7. A system comprising a parvovirus vector and a cell expressing the capsid proteins of parvovirus, wherein the parvovirus vector comprising a parvovirus DNA having a left terminus which comprises a parvovirus minimal origin of replication comprising CTWWTCA, wherein W is any nucleotide, and the parvovirus DNA is excisable from the parvovirus vector in a parvovirus-permissive cell, wherein the parvovirus DNA region coding for capsid proteins is partially or fully replaced by an exogeneous DNA, wherein the expression of the capsid proteins is controlled by a helper plasmid comprising an SV40 origin of replication and the cell expresses an SV40 large T antigen. 
     
     
       8. The system according to  claim 7 , wherein the DNA coding for the capsid proteins is under the control of the parvovirus promoter P38.