Analyzing method of a blood coagulation reaction

Abstract

An analyzing method of a blood coagulation reaction by detecting an optical change of a blood sample with an elapse of time, the method includes: setting at least one checkpoint or check region between a starting point of the blood coagulation reaction and the endpoint thereof; and monitoring a reaction state of the blood coagulation reaction at the checkpoint or in the check region to detect an abnormality of the blood coagulation reaction.

1. An analyzing method of a blood coagulation reaction by detecting an optical change of a blood sample with an elapse of time, the method comprising the steps of:
 setting a checkpoint or check region between the start of the reaction and the end of reaction, and monitoring a reaction state of the reaction by: 
 computing a check item at the checkpoint or check region; 
 comparing the check item with a predetermined threshold value; and 
 judging the presence or absence of abnormality of the blood coagulation reaction based on the compared result; 
 wherein the check item is selected from the group consisting of a first item, a second item, a third item and a fourth item, 
 the first item being a first time required for changing from a first optical detecting value to a second optical detecting value, 
 the second item being an amount of optical change in a predetermined time range, 
 the third item being a ratio between a second time required for changing from a third optical detecting value to a fourth optical detecting value and a third time required for changing from a fifth optical detecting value to a sixth optical detecting value, and 
 the fourth item being a fourth time required for reaching a predetermined optical detecting value. 
 
     
     
       2. The method of  claim 1 , wherein the coagulation reaction starts by mixing the blood sample with a blood coagulation reagent. 
     
     
       3. The method of  claim 1 , wherein the checkpoint and check region are shown based on a coagulation reaction curve represented by the optical change with the elapse of time. 
     
     
       4. The method of  claim 1 , wherein the predetermined threshold value is a first threshold value, and the judging step judges a presence or absence of abnormality of a reaction rate of fibrin formation stage in the blood coagulation reaction based on the compared result of the first item and the first threshold. 
     
     
       5. The method of  claim 1 , wherein the predetermined threshold value is a second threshold value, and the judging step judges a presence or absence of an initial stage in the blood coagulation reaction based on the compared result of the second item and the second threshold. 
     
     
       6. The method of  claim 1 , wherein the predetermined threshold value is a third threshold value, and the judging step judges a presence or absence of a drift in a coagulation reaction curve represented by the optical change with the elapse of time based on the compared result of the third item and the third threshold. 
     
     
       7. The method of  claim 1 , wherein the judging step judges whether or not the blood sample is an abnormal sample based on the judgment of the presence or absence of abnormality. 
     
     
       8. The method of  claim 1 , wherein, when the check item is the first item, a point exhibiting 50% of the total optical change amount exists between the first optical detecting value and the second optical detecting value. 
     
     
       9. The method of  claim 1 , wherein the predetermined time range is a time interval of the initial stage of the blood coagulation reaction. 
     
     
       10. The method of  claim 1 , wherein, when the check item is the third item, a point exhibiting 50% of the total optical change amount exists between the fifth optical detecting value and the sixth optical detecting value, and a point exhibiting 10% of the total optical change amount exists between the third optical detecting value and the fourth optical detecting value. 
     
     
       11. The method of  claim 1 , further comprising a step of displaying an error based on the compared result. 
     
     
       12. The method of  claim 1 , further comprising a step of measuring a blood coagulation time based on the blood coagulation reaction.