Method for producing L-lysine or L-arginine by using methanol-assimilating bacterium

Abstract

A DNA encoding a variant of a protein, the protein having a loop region and six hydrophobic helices and involved in secretion of L-lysine to the outside of a cell, wherein the DNA encodes a variant of a protein not containing the loop region and facilitates secretion of L-lysine, L-arginine or both of these L-amino acids to the outside of a methanol-assimilating bacterium when the DNA is introduced into the bacterium, specifically lysE24, is introduced into a Methylobacillus bacteria to improve L-amino acid productivity, especially L-lysine and L-arginine productivities.

1. A bacterium belonging to the genus  Methylobacillus , into which a DNA which is able to be expressed is introduced, and which has an ability to produce L-lysine or L-arginine, wherein said DNA encodes a protein comprising the amino acid sequence of SEQ ID NO: 10. 
     
     
       2. A method for producing L-lysine or L-arginine, comprising culturing the bacterium belonging to the genus  Methylobacillus  of  claim 1  in a medium to produce and accumulate L-lysine or L-arginine in culture, and collecting L-lysine or L-arginine from the culture. 
     
     
       3. The method for producing L-lysine or L-arginine according to  claim 2 , wherein the medium contains methanol. 
     
     
       4. The bacterium according to  claim 1 , wherein said DNA comprises the nucleotide sequence of SEQ ID NO: 9. 
     
     
       5. A method for producing L-lysine or L-arginine, comprising culturing the bacterium belonging to the genus  Methylobacillus  of  claim 4  in a medium to produce and accumulate L-lysine or L-arginine in culture, and collecting L-lysine or L-arginine from the culture. 
     
     
       6. The method for producing L-lysine or L-arginine according to  claim 5 , wherein the medium contains methanol.