Method for preparing xylitol with high yield using recycling microorganism

Abstract

Provided is a process for continuously producing xylitol in high yield and productivity using a vacuum microfiltration bioreactor containing a fermentation medium for a strain of the genus Candida , which includes: 5 300 g/l of xylose, 1 10 g/l of urea, 1 10 g/l of potassium diphosphate, 0.01 1 g/l of magnesium sulfate, 0.1-10 mg/l of MnSO 4 .4H 2 O, 0.1 10 mg/l of CoCl 2 .6H 2 O, 0.1 10 mg/l of NaMoO 4 .2H 2 O, 0.1 10 mg/l of ZnSO 4 .7H 2 O, 0.1 10 mg/l of AlCl 3 .6H 2 O, 0.1 10 mg/l of CuCl 2 .2H 2 O, 0.01-5 mg/l of H 3 BO 3 , 1-100 mg/l of FeSO 4 7H 2 O, 0.1-10 mg/l of ascorbic acid, 1-100 mg/l of biotin, 1-100 mg of choline, 1-200 mg/l of folic acid, 1-100 mg/l of inositol, 1-100 mg/l of nicotinic acid, 0.1-10 mg/l of p-aminobezoic acid, 1-100 mg/l of pantothenic acid, 0.1-10 mg/l of pyridoxine, 10-1,000 mg/l of riboflavin, and 1-100 mg/l of thiamine.

1. A chemically defined medium for fermentation culture of a strain of the genus  Candida , which comprises 5-300 g/l of xylose, 1-10 g/l of urea, 1-10g/l of potassium diphosphate, 0.01-1 g/l of magnesium sulfate, 0.1-10 mg/l of MnSO 4 .4H 2 O, 0.1-10mg/l of CoCl 2 .6H 2 O, 0.1-10 mg/l of NaMoO 4 .2H 2 O, 0.1-10 mg/l of ZnSO 4 .7H 2 O, 0.1-10 mg/l of AlCl 3 .6H 2 O, 0.1-10 mg/l of CuCl 2 .2H 2 O, 0.01-5 mg/l of H 3 BO 3 , 1-100 mg/l of FeSO 4 .7H 2 O, 0.1-10 mg/l of ascorbic acid, 1-100 mg/l of biotin, 1-100 mg/l of choline, and 0.1-10 mg/l of pyridoxine.