Method of examining specimen and specimen container to be used in the examination method

Abstract

A method of immunologically examining a specimen which comprises attaching a cap having a filter impregnated with a labeled antibody enclosed therein to a container body containing a diluted liquid specimen, pouring the diluted liquid specimen from the container into a test device, observing the reaction and thus examining the presence or absence of an analyte in the specimen. This examination method, whereby effects of differences among individual operators can be minimized and the occurrence of a nonspecific reaction can be prevented, is highly excellent in the reproducibility of the examination results and storage stability of a test reagent. A specimen container to be used in the above method to which a cap having a filter impregnated with a labeled antibody enclosed therein is attached. This container is appropriately usable as a member constituting a simplified diagnosis kit.

1. A method for immunologically examining a specimen, comprising the steps of:
 attaching a cap having a built-in filter impregnated with a labeled antibody to a container body that houses a diluted liquid specimen; 
 dispensing the diluted liquid specimen from the container through the built-in filter of the cap so as to react the diluted liquid specimen and the impregnated labeled antibody; 
 introducing the dispensed diluted liquid specimen to a test device and observing a reaction; and 
 determining a presence or absence of an analyte in the specimen. 
 
     
     
       2. The method according to  claim 1 , wherein the labeled antibody is a colloidal gold labeled antibody. 
     
     
       3. The method according to  claim 1 , wherein the analyte in the specimen is influenza virus. 
     
     
       4. A specimen container to be used in the examination method according to any one of  claims 1  to  3 , comprising a cap having a built-in, flow-through filter impregnated with a labeled antibody which is attached to a container body that is to house a diluted liquid specimen. 
     
     
       5. The specimen container according to  claim 4 , wherein the labeled antibody is a colloidal gold labeled antibody. 
     
     
       6. The specimen container according to  claim 4 , wherein the analyte in the specimen is influenza virus. 
     
     
       7. The specimen container according to  claim 4 , wherein the container is used as a component of a simplified diagnostic kit.