Lipoxygenase

Abstract

The invention provides sequence information of a microbial protein having lipoxy-genase activity and a method of producing the protein by recombinant DNA technology. More specifically, the inventors have isolated a gene encoding a lipoxygenase from Gaeu - mannomyces graminis , cloned it into an E. coli strain and sequenced it. A comparison shows less than 25% identity to known lipoxygenase sequences, the closest being human 15S li-poxygenase. The inventors have expressed the lipoxygenase recombinantly and found that the recombinant lipoxygenase is glycosylated.

1. An isolated polypeptide having lipoxygenase activity which:
 a) has at least 98% identity with the mature polypeptide of SEQ ID NO: 2; or 
 b) is encoded by the lipoxygenase-encoding part of the DNA sequence cloned into a plasmid present in  Escherichia coil  deposit number DSM 13586. 
 
     
     
       2. The polypeptide of  claim 1 , wherein the polypeptide has lipoxygenase activity and is encoded by the lipoxygenase-encoding part of the DNA sequence cloned into a plasmid present in  Escherichia coil  deposit number DSM 13586. 
     
     
       3. A dough composition comprising a polypeptide of  claim 1 . 
     
     
       4. A detergent composition comprising a polypeptide of  claim 1  and a surfactant. 
     
     
       5. The detergent composition of  claim 4 , wherein the surfactant is an anionic surfactant. 
     
     
       6. The detergent composition of  claim 4 , wherein the surfactant is a linear alkyl benzenesulfonate. 
     
     
       7. The isolated polypeptide of  claim 1 , wherein the polypeptide comprises the amino acid sequence of SEQ ID NO:2. 
     
     
       8. The isolated polypeptide of  claim 1 , wherein the polypeptide consists of the amino acid sequence of SEQ ID NO:2. 
     
     
       9. An isolated polypeptide having lipoxygenase activity which is encoded by the lipoxygenase-encoding part of the DNA sequence cloned into a plasmid present in  Escherichia coli  deposit number DSM 13586. 
     
     
       10. The isolated polypeptide of  claim 9 , wherein the polypeptide comprises the amino acid sequence of SEQ ID NO:2. 
     
     
       11. The isolated polypeptide of  claim 9 , wherein the polypeptide consists of the amino acid sequence of SEQ ID NO:2. 
     
     
       12. A dough composition comprising the polypeptide of  claim 9 . 
     
     
       13. A detergent composition comprising the polypeptide of  claim 9  and a surfactant.