Sulfonate compound and fluorescent probe using the same

Abstract

The present invention provides a sulfonate compound including a structure represented by a general formula (I) below. In the formula (I), an atomic group A-O is an atomic group that forms a fluorescent compound upon cleavage of a covalent bond with a sulfonyl group. There may be one or plural atomic groups B—SO 3 — bound to an atomic group A. B is a ring substituted by one or plural electron-withdrawing groups. The electron-withdrawing group includes at least one selected from the group consisting of halogens, a carboxyl group, a carbamoyl group, a straight or branched alkylcarbamoyl group, a straight or branched alkanoyl group, a straight or branched alkoxycarbonyl group, a straight or branched alkyl halide group, and —NR 3 + group (the three Rs each denote a hydrogen atom or a straight or branched alkyl group and may be the same or different). When there are plural Bs, the Bs may be the same or different.

1. A sulfonate compound represented by a formula (I) below, 
       
         
           
           
               
               
           
         
         where an atomic group A-O is an atomic group that forms a fluorescent compound (excluding hydroxycoumarin and derivatives thereof) upon cleavage of a covalent bond with a sulfonyl group, 
         there may be one or plural atomic groups B—SO 3 — bound to an atomic group A, 
         A is represented by a formula (X-1) or (X-2) below, 
       
       
         
           
           
               
               
           
         
         where, in the above formulae, X 1 , X 2 , X 3  and X 4  each denote a hydrogen atom or halogen atom, and may be the same or different, and 
         an atomic group B is at least one selected from the group consisting of a pentafluorophenyl group, a 2,4,6-trifluorophenyl group, a 2,4,6-tris(trifluoromethyl)phenyl group, and a 2,4-bis(trifluoromethyl)phenyl group. 
       
     
     
       2. The sulfonate compound according to  claim 1 , which is represented by any one of formulae 1 and 2 below, 
       
         
           
           
               
               
           
         
         where X 1  to X 4  in the formulae 1 and 2 each denote a hydrogen atom or a halogen and may be the same or different. 
       
     
     
       3. A method for producing the sulfonate compound according to  claim 1 , comprising the step of combining a compound represented by a formula (II) below and a compound represented by a formula (III) below, 
       
         
           
           
               
               
           
         
         where X 5  in the formula (III) is a halogen, and A and B are the same as A and B in the formula (I).