Gene promoters

Abstract

The invention is directed to a method for obtaining a promoter from an organism using a proteomics approach, isolated promoters from fungal species and uses of the isolated promoters.

1. An isolated hex 1 promoter of  Trichoderma reesei , having a nucleotide sequence substantially as shown in SEQ ID NO: 1, a nucleotide sequence which hybridizes to the nucleotide sequence as shown in SEQ ID NO: 1 under stringent conditions, or having a functional part of the sequence shown in SEQ ID NO: 1 which is capable of controlling expression of a gene when in a fungal species. 
     
     
       2. The hex1 promoter according to  claim 1  having the nucleotide sequence substantially as shown in SEQ ID NO: 1. 
     
     
       3. The hex1 promoter according to  claim 1  having a nucleotide sequence which hybridizes to the sequence of SEQ ID NO:1 under high stringency hybridization conditions. 
     
     
       4. A vector or transformed cell which contains the isolated hex1 promoter according to  claim 1 . 
     
     
       5. The vector according to  claim 4  selected from the group consisting of nucleic acid vector, plasmid, and viral vector. 
     
     
       6. A recombinant DNA construct comprising the isolated hex1 promoter according to  claim 1  operably linked to a gene. 
     
     
       7. The DNA construct according to  claim 6  wherein the gene encodes a protein. 
     
     
       8. The DNA construct according to  claim 7  wherein the protein is selected from the group consisting of an hydrolytic enzyme, and molecule suitable for therapeutic or pharmaceutical use. 
     
     
       9. The DNA construct according to  claim 8  wherein the hydrolytic enzyme is selected from the group consisting of phytases, cellulases, xylanases, beta-glucanases, amylases, lipases, and proteases. 
     
     
       10. The DNA construct according to  claim 8  wherein the molecule suitable for therapeutic or pharmaceutical use is selected from the group consisting of proteins, antibodies, growth factors, growth inhibitors, and tissue activators. 
     
     
       11. An expression system containing the DNA construct according to  claim 7 .