Latex reagent for adiponectin analysis and method of adiponectin analysis

Abstract

A latex reagent for analyzing adiponectin, comprising a suspension of latex particles carrying a substance which specifically binds to adiponectin, is disclosed. Further, a method for analyzing adiponectin, comprising (1) obtaining a biological liquid possibly containing adiponectin, and (2) bringing the biological liquid, while maintaining the state in which the biological liquid is obtained, into contact with a suspension of latex particles carrying a substance which specifically binds to adiponectin, and optically analyzing a degree of latex-particles-agglutination, is disclosed. According to the latex reagent and the method for analyzing adiponectin, a predilution or pretreatment of the biological liquid to be analyzed is not necessary. Further, the analysis can be performed rapidly and conveniently, and facilities therefor are not limited.

1. A latex reagent for quantitatively measuring adiponectin, comprising a suspension of latex particles carrying an anti-adiponectin polyclonal antibody that binds to native adiponectin. 
     
     
       2. The latex reagent according to  claim 1 , wherein the latex particles do not carry an anti-adiponectin monoclonal antibody. 
     
     
       3. A method for quantitatively measuring native adiponectin, comprising the steps of:
 (1) obtaining a biological liquid possibly containing adiponectin, and 
 (2) bringing the biological liquid, without pretreatment of said liquid to obtain monomeric adiponectin or predilution, into contact with a suspension of latex particles carrying an anti-adiponectin polyclonal antibody that binds to native adiponectin, and optically analyzing a degree of latex-particles-agglutination, wherein said degree of latex-particle agglutination correlates to the level of adiponectin in said liquid. 
 
     
     
       4. The method according to  claim 3 , wherein the latex particles do not carry an anti-adiponectin monoclonal antibody. 
     
     
       5. A method for quantitatively measuring the level of native adiponectin in a biological liquid, consisting of the steps of:
 (1) obtaining a biological liquid possibly containing adiponectin; and 
 (2) bringing the biological liquid, without predilution or other pretreatment, into contact with a suspension of latex particles carrying an anti-adiponectin polyclonal antibody that binds to native adiponectin, and optically analyzing a degree of latex-particle-agglutination, wherein said degree of latex-particle agglutination correlates to the level of adiponectin in said liquid.