Polynucleotide encoding an IL-28A polypeptide

Abstract

Homogeneous preparations of IL-28A, IL-28B, and IL-29 have been produced by mutating one or more of the cysteine residues in the polynucleotide sequences encoding the mature proteins. The cysteine mutant proteins can be shown to either bind to their cognate receptor or exhibit biological activity. One type of biological activity that is shown is an antiviral activity.

1. An isolated polynucleotide encoding a polypeptide wherein the encoded polypeptide comprises amino acid residues 1-175 of SEQ ID NO:19. 
     
     
       2. The polynucleotide of  claim 1  wherein the encoded polypeptide has anti-hepatitis activity. 
     
     
       3. The polynucleotide of  claim 2  wherein the encoded polypeptide has anti-hepatitis B activity. 
     
     
       4. The polynucleotide of  claim 2  wherein the encoded polypeptide has anti-hepatitis C activity. 
     
     
       5. An isolated polynucleotide comprising nucleotides 1-528 of SEQ ID NO:18. 
     
     
       6. An expression vector comprising the following operably linked elements:
 a transcription promoter; 
 a DNA segment encoding a polypeptide comprising amino acid residues 1-175 of SEO ID NO:19; and 
 a transcription terminator. 
 
     
     
       7. A cultured cell comprising the expression vector of  claim 6 , wherein the cell expresses the polypeptide encoded by the DNA segment. 
     
     
       8. A method of producing a polypeptide comprising:
 culturing a cell comprising the expression vector of  claim 6 , wherein the cell expresses the polypeptide encoded by the DNA segment; and 
 recovering the expressed polypeptide.