Non-immunostimulatory antibody and compositions containing the same

Abstract

The present invention relates to a non-immunostimulatory antibody which lacks antibody-dependent cell-mediated cytotoxicity, Fc gamma receptor binding and complement-mediated cytotoxicity. In some embodiments, the antibody contains a modified immunoglobulin G2 (IgG2) Fc region with at least one substitution in the B/C loop, FcRn binding domain, and the F/G loop. The antibody of the invention is useful in the preparation of therapeutic antibodies and pharmaceutical compositions and kits containing the same.

1. An isolated non-immunostimulatory antibody comprising glutamine at residue 268, leucine at residue 309, serine at residue 330 and serine at residue 331 according to the Kabat numbering system,
 wherein the amino acid sequence of the Fc region of said antibody comprises at least 93% of the amino acid sequence of human immunoglobulin G2 (IgG2) Fc region, and 
 wherein said antibody lacks antibody-dependent cell-mediated cytotoxicity, Fc gamma receptor binding and complement-mediated cytotoxicity. 
 
     
     
       2. The isolated non-immunostimulatory antibody of  claim 1 , wherein the amino acid sequence of the Fc region of said antibody is set forth in SEQ ID NO:1. 
     
     
       3. A pharmaceutical composition comprising the isolated non-immunostimulatory antibody of  claim 1 . 
     
     
       4. A kit comprising the isolated non-immunostimulatory antibody of  claim 1 .