Immunoreactive 38-KDA ferric binding protein of ehrlichia canis and uses thereof

Abstract

The present invention is directed to the cloning, sequencing, expression, and characterization of an immunoreactive ferric binding protein (Fbp) (38-kDa) protein of Ehrlichia canis encoded by a polynucleotide therefor. In particular embodiments, the protein is employed in an immunogenic composition, such as a vaccine. Methods to induce an immune reaction in an individual with compositions of the invention are provided.

1. An isolated polynucleotide, comprising:
 a) SEQ ID NO:2; or 
 b) a polynucleotide that is at least about 95% identical to SEQ ID NO:2. 
 
     
     
       2. The polypeptide of  claim 1 , comprised in a pharmaceutically acceptable excipient. 
     
     
       3. The polypeptide of  claim 1 , comprised in a pharmaceutical composition suitable as an immunogenic composition. 
     
     
       4. A method of inducing an immune response in an individual, comprising the step of administering to the individual a therapeutically effective amount of the polypeptide of  claim 1 , wherein said administering induces the immune response. 
     
     
       5. The polypeptide of  claim 1 , wherein the polypeptide of b) is a polypeptide that is at least 97% identical to SEQ ID NO:2. 
     
     
       6. The polypeptide of  claim 1 , wherein the polypeptide of b) is a polypeptide that is at least 99% identical to SEQ ID NO:2.