Graft prosthesis devices containing renal capsule collagen

Abstract

The invention provides tissue graft compositions comprising collagen-based extracellular matrices derived from renal capsules of warm-blooded vertebrates. The invention further provides a process of harvesting and purifying a renal capsule to provide an extracellular matrix material having beneficial use as a tissue graft and/or cell growth material.

1. A method for preparing an extracellular matrix tissue material, comprising: providing a renal capsule tissue and decellularizing said renal capsule tissue to form the extracellular matrix tissue material, and sterilizing the extracellular matrix tissue material after said decellularizing, wherein the extracellular matrix tissue material contains residual bioactive proteins after said sterilizing, and wherein the residual bioactive proteins include Fibroblast Growth Factor-2, Vascular Endothelial Growth Factor, or a combination thereof. 
     
     
       2. The method of  claim 1  where in said renal capsule tissue is from a warm-blooded vertebrate. 
     
     
       3. The method of  claim 2  wherein said warm-blooded vertebrate is selected from a group consisting of porcine, bovine and ovine. 
     
     
       4. The method of  claim 2  wherein said warm-blooded vertebrate is a pig. 
     
     
       5. The method of  claim 1  wherein said decellularizing comprises exposing said renal capsule tissue to a decellularization agent comprising an oxidizing agent. 
     
     
       6. The method of  claim 5  wherein said oxidizing agent is diluted in an aqueous alcohol. 
     
     
       7. The method of  claim 5  wherein said agent is a peroxy compound. 
     
     
       8. The method of  claim 7  wherein said peroxy compound is peracetic acid. 
     
     
       9. The method of  claim 1  wherein said extracellular matrix tissue material comprises a renal capsule extracellular matrix structure having a tensile strength of at least about 4 MPa. 
     
     
       10. The method of  claim 1 , further comprising lyophilizing the extracellular matrix tissue material after said decellularizing. 
     
     
       11. The method of  claim 1 , wherein said extracellular matrix tissue material is in the form of a powder, a fluidized composition, a sheet, a tube, a pouch, a multi-ply construct, or a single-ply construct. 
     
     
       12. The method of  claim 11 , wherein said extracellular matrix tissue material is in the form of a powder. 
     
     
       13. The method of  claim 11 , wherein said extracellular matrix tissue material is in the form of a multi-ply construct. 
     
     
       14. The method of  claim 1 , wherein the residual bioactive proteins include Fibroblast Growth Factor-2. 
     
     
       15. The method of  claim 1 , wherein the residual bioactive proteins include Vascular Endothelial Growth Factor. 
     
     
       16. The method of  claim 1 , wherein the residual bioactive proteins further include one or more glycosaminoglycans, glycoproteins, proteoglycans, cytokines, growth factors, or any combination thereof.