L-methionine producing microorganism and method of producing L-methionine using the microorganism

Abstract

Disclosed herein are a method of preparing an L-methionine production strain by overexpressing proteins involved in L-methionine biosynthesis in an L-threonine production strain, a strain prepared by the method, and a method of producing L-methionine using the strain.

1. An L-methionine production bacterial strain, wherein the strain has the ability to produce L-threonine, and is transformed with one or more recombinant vectors expressing (a) threonine dehydratase; and (b) at least one of the proteins selected from a group consisting of O-succinylhomoserine lyase, cystathionine beta lyase, 5,10-methylenetetrahydrofolate reductase and serine hydroxymethyltransferase. 
     
     
       2. The L-methionine production strain as set forth in  claim 1 , wherein the strain is transformed with a recombinant vector expressing threonine dehydratase, O-succinylhomoserine lyase and cystathionine beta lyase. 
     
     
       3. A method of producing L-methionine, in which the L-methionine production strain of  claim 1  is fermented. 
     
     
       4. The L-methionine production strain as set forth in  claim 1 , wherein the strain is transformed with a recombinant vector expressing threonine dehydratase, 5,10-methylenetetrahydrofolate reductase and serine hydroxymethyltransferase. 
     
     
       5. The L-methionine production strain as set forth in  claim 1 , wherein the strain is transformed with a recombinant vector expressing threonine dehydratase, O-succinylhomoserine lyase, cystathionine beta lyase. 5,10-methylenetetrahydrofolate reductase and serine hydroxymethyltransferase. 
     
     
       6. The L-methionine production strain as set forth in  claim 1 , wherein the strain does not require methionine for growth. 
     
     
       7. The L-methionine production strain as set forth in  claim 2 , wherein the strain does not require methionine for growth. 
     
     
       8. The L-methionine production strain as set forth in  claim 4 , wherein the strain does not require methionine for growth. 
     
     
       9. The L-methionine production strain as set forth in  claim 5 , wherein the strain does not require methionine for growth. 
     
     
       10. The L-methionine production strain as set forth in  claim 1 , wherein the strain is prepared by transforming  Escherichia coli  MF001(KCCM-10568) with one or more recombinant vectors expressing (a) threonine dehydratase; and (b) at least one of the proteins selected from a group consisting of O-succinylhomoserine lyase, cystathionine beta lyase, 5,10-methylenetetrahydrofolate reductase and serine hydroxymethyltransferase. 
     
     
       11. A method of producing L-methionine, in which the L-methionine production strain of  claim 2  is fermented. 
     
     
       12. A method of producing L-methionine, in which the L-methionine production strain of  claim 4  is fermented. 
     
     
       13. A method of producing L-methionine, in which the L-methionine production strain of  claim 5  is fermented. 
     
     
       14. A method of producing L-methionine, in which the L-methionine production strain of  claim 6  is fermented. 
     
     
       15. A method of producing L-methionine, in which the L-methionine production strain of  claim 7  is fermented.