Method of producing double low restorer lines of Brassica napus having a good agronomic value

Abstract

The invention relates to a method of producing a double low restorer line of Brassica napus for Ogura cytoplasmic male sterility (cms) presenting a radish introgression carrying the Rfo restorer gene deleted of the radish Pgi-2 allele and recombined with the Pgi-2 gene from Brassica oleracea , and having a good agronomic value characterized by female fertility, a good transmission rate of Rfo and a high vegetative vigour. The invention relates also to a method of forming Brassica napus hybrid seeds and progeny thereof and to the use of markers for selection.

1. A method for characterising recombined restorer lines of  Brassica napus  for Ogura cms presenting a Rfo insertion deleted of the radish Pgi-2 allele and recombined with the Pgi-2 gene from  Brassica oleracea , and having an agronomic value characterised by female fertility, transmission rate of Rfo and vegetative vigour, comprising a step wherein the presence of the five markers PGIol, PGIUNT, PGIint, BolJon and CP418 is detected in said recombined restorer lines and wherein said markers comprise the following sequences:
 PGIol marker: SEQ ID NO:1; 
 PGIUNT marker: SEQ ID NO:2; 
 PGIint marker: SEQ ID NO:3; 
 BolJon marker: SEQ ID NO:4; and 
 CP418 marker: SEQ ID NO:5.