Anti-heparan sulfate antibody, method for detection of heparan sulfate, and kit for detection of heparan sulfate

Abstract

An antibody which reacts with N-acetylheparosan and heparan sulfate that is derived from bovine kidney but does not substantially react with heparan sulfate derived from a murine Engelbreath-Holm-Swarn tumor tissue, the antibody being produced with a hybridoma which is prepared using a substance composed of a protein and N-acetylheparosan bound to the protein.

1. A hybridoma which has the Deposition number of FERM BP-10534, FERM BP-10535, or FERM BP-10536. 
     
     
       2. An isolated monoclonal antibody, which is produced by the hybridoma having Deposition number of FERM BP-10534, FERM BP-10535, or FERM BP-10536. 
     
     
       3. A kit for detecting heparan sulfate or N-acetylheparosan in a sample, comprising at least one antibody according to  claim 2 . 
     
     
       4. A method of detecting heparan sulfate or N-acetylheparosan in a sample, comprising:
 contacting the sample with the antibody according to  claim 2 ; and 
 detecting the antibody bound to the heparan sulfate or N-acetylheparosan. 
 
     
     
       5. The detecting method according to  claim 4 , wherein the sample is derived from a source selected from the group consisting of body fluids, cells, tissues, and cultures of cells or microorganisms.