Isolating human antibodies

Abstract

The invention is drawn to a method of isolating human antibodies.

1. A method for isolating human antibodies, comprising:
 a) obtaining B-cells; 
 b) purifying said B-cells to provide purified B-cells; 
 c) contacting said purified B-cells with at least one labeled target at a number of target concentrations to identify a concentration at which the target concentration is limiting, thereby selecting for purified B-cells that bind tightly to the target; 
 d) collecting said B-cells bound to said target; and 
 e) distinguishing, separating and identifying the antibody to which said B-cells have bound to said target, 
 wherein said at least one labeled target has a target concentration is between about 1 micromolar (1e-6M) and about 1 picomolar (1e-12M). 
 
     
     
       2. The method of  claim 1 , wherein the target concentration is between about 1e-7 M and about 1e-11 M.