Canine respiratory coronavirus (CRCV) spike protein

Abstract

A canine respiratory coronavirus (CRCV) that is present in the respiratory tract of dogs with canine infectious respiratory disease and which has a low level of homology to the enteric canine coronavirus, but which has a high level of homology to all bovine coronavirus strains (e.g., Quebec and LY138) and human coronavirus strain OC43.

1. An isolated coronavirus S protein having at least 97% amino acid sequence identity with SEQ ID NO:4. 
     
     
       2. An isolated polynucleotide that encodes the protein according to  claim 1 , or the complete complement thereof. 
     
     
       3. The polynucleotide according to  claim 2  comprising SEQ ID NO:3. 
     
     
       4. A vector comprising the polynucleotide of  claim 2 . 
     
     
       5. An isolated host cell comprising the vector of  claim 4 . 
     
     
       6. An isolated host cell according to  claim 5  wherein the vector is an expression vector comprising a eukaryotic promoter operatively linked to the polynucleotide, and wherein the host cell is a eukaryotic host cell. 
     
     
       7. The isolated coronavirus protein of  claim 1 , wherein said protein comprises at least one of: V at position 103; V at position 118; D at position 166; M at position 171; K at position 179; P at position 192; S at position 210; H at position 235; F at position 267; F at position 388; M at position 407; S at position 436; I at position 440; I at position 447; F at position 501; Y at position 525; N at position 528; L at position 540; K at position 582; G at position 608; G at position 692; S at position 695; W at position 757; G at position 758; Q at position 763; T at position 769; P at position 786; H at position 792; R at position 818; P at position 827; V at position 828; F at position 887; D at position 933; F at position 977; T at position 1011; S at position 1018; K at position 1063; L at position 1256; and M at position 1257, wherein the amino acid positions are from SEQ ID NO:4. 
     
     
       8. The isolated coronavirus protein of  claim 1  comprising the amino acid sequence of SEQ ID NO:4.