Isolated genomic nucleic acid molecules obtainable from chromosome 1p21-p13 that encode human RhoC

Abstract

The invention is directed to an isolated genomic nucleic acid molecule fragment that encodes human RhoC, vectors and hosts containing the fragment and fragments hybridizing to noncoding regions as well as antisense oligonucleotides to these fragments. The invention is further directed to methods of using these fragments to obtain human RhoC and to diagnose, treat, prevent and/or ameliorate a pathological disorder.

1. An isolated genomic nucleic acid molecule, said nucleic acid molecule obtainable from human chromosome 1, selected from the group consisting of:
 a) an isolated polynucleotide consisting of a nucleic acid sequence which is at least 99% identical to the full length polynucleotide of SEQ ID NO:2, which encodes a polypeptide which is at least 99% identical to the amino acid sequence of SEQ ID NO: 1, 
 b) an isolated polynucleotide fragment of (a) which comprises at least nucleotides 3959-6218 of SEQ ID NO:2, which encodes a polypeptide which is at least 99% identical to the amino acid sequence of SEQ ID NO: 1, and 
 c) a full complement of (a) or (b). 
 
     
     
       2. A nucleic acid construct comprising an isolated genomic nucleic acid molecule, said nucleic acid molecule obtainable from human chromosome 1, selected from the group consisting of:
 a) an isolated polynucleotide consisting of a nucleic acid sequence which is at least 99% identical to the full length polynucleotide of SEQ ID NO:2, which encodes a polypeptide which is at least 99% identical to the amino acid sequence of SEQ ID NO: 1, 
 b) an isolated polynucleotide fragment of (a) which comprises at least nucleotides 3959-6218 of SEQ ID NO:2, which encodes a polypeptide which is at least 99% identical to the amino acid sequence of SEQ ID NO: 1, and 
 c) a full complement of (a) or (b). 
 
     
     
       3. An expression vector comprising an isolated genomic nucleic acid molecule, said nucleic acid molecule obtainable from human chromosome 1, selected from the group consisting of:
 a) an isolated polynucleotide consisting of a nucleic acid sequence which is at least 99% identical to the full length polynucleotide of SEQ ID NO:2, which encodes a polypeptide which is at least 99% identical to the amino acid sequence of SEQ ID NO: 1, 
 b) an isolated polynucleotide fragment of (a) which comprises at least nucleotides 3959-6218 of SEQ ID NO:2, which encodes a polypeptide which is at least 99% identical to the amino acid sequence of SEQ ID NO: 1, and 
 c) a full complement of (a) or (b). 
 
     
     
       4. A recombinant host cell comprising an isolated genomic nucleic acid molecule, said nucleic acid molecule obtainable from human chromosome 1, selected from the group consisting of:
 a) an isolated polynucleotide consisting of a nucleic acid sequence which is at least 99% identical to the full length polynucleotide of SEQ ID NO:2, which encodes a polypeptide which is at least 99% identical to the amino acid sequence of SEQ ID NO: 1, 
 b) an isolated polynucleotide fragment of (a) which comprises at least nucleotides 3959-6218 of SEQ ID NO:2, which encodes a polypeptide which is at least 99% identical to the amino acid sequence of SEQ ID NO: 1, and 
 c) a full complement of (a) or (b). 
 
     
     
       5. A method for obtaining a polypeptide encoded by a nucleic acid molecule obtainable from human chromosome 1, said polypeptide consisting of human RhoC or a variant thereof comprising:
 (a) culturing the recombinant host cell of  claim 4  under conditions that provide for the expression of said polypeptide and 
 (b) recovering said expressed polypeptide. 
 
     
     
       6. A composition comprising the nucleic acid molecule of  claim 1  and a pharmaceutically acceptable carrier. 
     
     
       7. A method for detecting the presence of the nucleic acid molecule of  claim 1  in a sample, comprising contacting the sample with a polynucleotide probe comprising at least 20 contiguous nucleotides that hybridizes to said nucleic acid molecule under stringent conditions and determining whether the polynucleotide probe binds to said nucleic acid molecule in the sample. 
     
     
       8. The nucleic acid molecule of  claim 1 , wherein said sequence encodes Rho C protein depicted in SEQ ID NO:1. 
     
     
       9. A kit comprising the nucleic acid molecule of  claim 1 . 
     
     
       10. A solid support comprising the nucleic acid molecule of  claim 1 . 
     
     
       11. The solid support of  claim 10 , wherein said solid support is a microarray.