Fluorescent probe and method of measuring hypochlorite ion

Abstract

A compound represented by the following general formula (II): wherein R 21 represents hydrogen atom or one to four monovalent substituents substituting on the benzene ring, and when R 21 represents two or more substituents, the substituents may be the same or different; R 22 , R 24 , R 25 , and R 27 independently represent hydrogen atom, or a monovalent substituent; R 23 represents —OH or —N(R 28 )(R 29 ) (wherein R 28 and R 29 independently represent an alkyl group which may be substituted); R 26 represents —N(R 30 )(R 31 ) (wherein R 30 and R 31 independently represent an alkyl group which may be substituted); n represents an integer of 1 to 3; and Y represents —S— or —O—, which is useful for selective measurement of hypochlorite ion.

1. A compound represented by the formula (I) or a salt thereof: 
       
         
           
           
               
               
           
         
         wherein R 1  represents hydrogen atom or one to four monovalent substituents substituting on the benzene ring, and when R 1  represents two or more substituents, the substituents may be the same or different; R 2 , R 4 , R 5 , and R 7  independently represent hydrogen atom, or a monovalent substituent; R 3  is ═N + (R 8 )(R 9 ).M − , wherein R 8  and R 9  independently represent an alkyl group which may be substituted, and M −  represents a counter ion; R 6  represents —N(R 10 )(R 11 ), wherein R 10  and R 11  independently represent an alkyl group which may be substituted; n represents an integer of 1 to 3; and X is —SH. 
       
     
     
       2. A compound represented by the formula (I) or a salt thereof: 
       
         
           
           
               
               
           
         
         wherein R 1  represents hydrogen atom or one to four monovalent substituents substituting on the benzene ring, and when R 1  represents two or more substituents, the substituents may be the same or different; R 2 , R 4 , R 5 , and R 7  independently represent hydrogen atom, or a monovalent substituent; R 3  is ═O; R 6  represents —OH; n represents an integer of 1 to 3; and X is —OH. 
       
     
     
       3. A method for measuring hypochlorite ion, which comprises the following:
 (a) contacting a compound represented by the following formula (II) or a salt thereof: 
 
       
         
           
           
               
               
           
         
         wherein R 21  represents hydrogen atom or one to four monovalent substituents substituting on the benzene ring, and when R 21  represents two or more substituents, the substituents may be the same or different; R 22 , R 24 , R 25 , and R 27  independently represent hydrogen atom, or a monovalent substituent; R 23  is —N(R 28 )(R 29 ), wherein R 28  and R 29  independently represent an alkyl group which may be substituted; R 26  represents —N(R 30 )(R 31 ), wherein R 30  and R 31  independently represent an alkyl group which may be substituted; n represents an integer of 1 to 3; and Y is —S—, with a sample, and 
         (b) measuring fluorescence generated from a compound generated by a reaction with hypochlorite ion in the sample.