Spirolactam targeting compounds and related compounds

Abstract

Spirolactam targeting compounds, related compounds, uses of such compounds, and methods of making such compounds are disclosed.

What we claim is: 
     
       1. A compound represented by Structure XIV: 
       
         
           
           
               
               
           
         
         wherein Z is CH 2 CH 2 NH, and wherein FL is a fluorophore selected from the group consisting of: 
       
       
         
           
           
               
               
           
         
       
     
     
       2. A composition comprising a polypeptide having a tetracysteine tag, and the compound of  claim 1  conjugated to the polypeptide via the tetracysteine tag. 
     
     
       3. A method of imaging a sample, the method comprising:
 providing a sample comprising a polypeptide having a tetracysteine tag; 
 contacting the sample with a compound of  claim 1 ; and 
 detecting emission from the compound to image the sample. 
 
     
     
       4. The method of  claim 3 , wherein the sample comprises a cell expressing the polypeptide having a tetracysteine tag.