Nucleotide sequences

Abstract

The present invention relates to the field of cancer therapy. More specifically, the invention relates to use of certain nucleotide sequences for the treatment of cancer.

1. A small interfering ribonucleic acid (siRNA) for inhibiting the expression of a protein encoded by SEQ ID 17 or an isoform thereof in a cell and polypeptide comprising SEQ ID 18, wherein the siRNA comprises at least 2 sequences that are complementary to each other and wherein a sense strand comprises a first sequence and an anti-sense strand comprises a second sequence comprising a region of complementarity, which is substantially complementary to at least a part of an mRNA encoding a nucleotide sequence from SEQ ID 17, wherein the said siRNA upon contact with a cell expressing protein encoded by SEQ ID 17 or an isoform thereof or expression of polypeptide comprising SEQ ID 18 in a cell and further wherein said first sequence is SEQ ID 13. 
     
     
       2. A cell comprising the siRNA of  claim 1 . 
     
     
       3. A vector comprising the siRNA of  claim 1 . 
     
     
       4. A pharmaceutical composition inhibiting expression of protein encoded by SEQ ID 17 or an isoform thereof in a cell, comprising the siRNA of  claim 1  together with a cellular uptake enhancing peptide segment or agent. 
     
     
       5. A method of inhibiting cancerous cellular growth/proliferation of cells expressing a protein encoded by SEQ ID 17, an isoform thereof or a polypeptide comprising SEQ ID 18, said method comprising the step of delivering to the cells the composition of  claim 4 . 
     
     
       6. A method of causing cell death in cells expressing a protein encoded by SEQ ID 17, an isoform thereof or a polypeptide comprising SEQ ID 18, said method comprising the step of delivering to the cell a composition comprising small interfering ribonucleic acid (siRNA) for inhibiting the expression of said protein encoded by SEQ ID 17, said isoform thereof or said polypeptide comprising SEQ ID 18 in a cell, wherein the siRNA comprises at least 2 sequences that are complementary to each other and wherein a sense strand comprises a first sequence and an anti-sense strand comprises a second sequence comprising a region of complementarity, which is substantially complementary to at least a part of an mRNA encoding a polynucleotide sequence from SEQ ID 17, together with an appropriate cellular uptake-enhancing peptide segment or agent and further wherein said first sequence is SEQ ID 13. 
     
     
       7. The method of  claim 6  for treatment of cancer, comprising delivery of a therapeutically effective amount of said siRNA to a subject in need thereof. 
     
     
       8. The method of  claim 7 , wherein said cancerous tissue is mammalian lung cancer tissue. 
     
     
       9. The method of  claim 7  wherein said cancerous tissue is selected from the group consisting of ovary, breast, lung, cervix, colon, liver, prostrate, skin, uterus, kidney, urinary bladder, endometrial, bone, pancreas, rectum, pharynx, vulva, placenta, brain, testis, eye, and stomach. 
     
     
       10. A method of inhibiting expression of a protein encoded by SEQ ID 17, an isoform thereof or a polypeptide comprising SEQ ID 18 in a cancer cell, said method comprising the step of delivering to the cell the composition of  claim 4 . 
     
     
       11. The method of  claim 10  wherein said cancer cell is selected from the group consisting of ovary, breast, lung, cervix, colon, liver, prostrate, skin, uterus, kidney, urinary bladder, endometrial, bone, pancreas, rectum, pharynx, vulva, placenta, brain, testis, eye, and stomach. 
     
     
       12. A method of reducing cell viability of cancer cells expressing a protein encoded by SEQ ID 17, an isoform thereof or a polypeptide comprising SEQ ID 18, said method comprising the step of delivering to the cell a composition comprising small interfering ribonucleic acid (siRNA) for inhibiting the expression of said protein encoded by SEQ ID 17, said isoform thereof or said polypeptide comprising SEQ ID 18 in a cell, wherein the siRNA comprises at least 2 sequences that are complementary to each other and wherein a sense strand comprises a first sequence and an anti-sense strand comprises a second sequence comprising a region of complementarity, which is substantially complementary to at least a part of an mRNA encoding a polynucleotide sequence from SEQ ID 17, together with an appropriate cellular uptake-enhancing peptide segment or agent and further wherein said first sequence is SEQ ID 13. 
     
     
       13. The method of  claim 12  wherein said cancer cells are selected from the group consisting of ovary, breast, lung, cervix, colon, liver, prostrate, skin, uterus, kidney, urinary bladder, endometrial, bone, pancreas, rectum, pharynx, vulva, placenta, brain, testis, eye, and stomach.