Family 8 enzymes with xylanolytic activity

Abstract

The present invention describes a method to improve the properties of a dough and/or a baked product by adding a bread or dough-improving agent containing a enzyme with xylanolytic activity belonging to glycoside hydrolases family 8. Preferred enzymes are the psychrophilic xylanase from Pseudoalteromonas haloplanktis and the mesophilic xylanase Y from Bacillus halodurans C-125.

1. An isolated psychrophilic Family 8 glycoside hydrolase enzyme with xylanolytic activity which is obtained from  Pseudoalteromonas haloplanktis , and which comprises the amino acid sequence of SEQ ID NO 7. 
     
     
       2. The isolated enzyme of  claim 1 , which has a molecular weight of about 46000 Da, an isoelectric point of approximately 9.5, an optimum temperature of about 35° C. and an optimum pH between about 5.3 and 8. 
     
     
       3. The isolated enzyme of  claim 1 , which hydrolyses the substrate with inversion of configuration. 
     
     
       4. The isolated enzyme of  claim 1 , which is obtained from  Pseudoalteromonas haloplanktis  TAH3a. 
     
     
       5. A composition comprising the enzyme of  claim 1 . 
     
     
       6. A composition comprising the enzyme of  claim 2 .