Luminogenic and fluorogenic compounds and methods to detect molecules or conditions

Abstract

A method to detect the presence or amount of at least one molecule in a sample which employs a derivative of luciferin or a derivative of a fluorophore is provided. Compounds and compositions for carrying out the methods of the invention are also provided.

What is claimed is: 
     
       1. A compound according to formula VI: 
       
         
           
           
               
               
           
         
         wherein R 1  is —NR N1 R N2 ; wherein R N1  is H, C 3-8 alkyl which may be substituted with hydroxyl, halo, or amino, or —CH 2 -A 1  or —CH 2 -A 2 ; 
         R N2  is C 3-8 alkyl which may be substituted with hydroxyl, halo, or amino or —CH 2 -A 1  or —CH 2 -A 2 ; or 
         A 1  is C 6-10 aryl or C 6-10 heteroaryl, which may be substituted with hydroxyl, halo, or amino; 
         A 2  is C 6  aryl or C 6  heteroaryl, which may be substituted with hydroxyl, halo, or amino; and only one of R N1  or R N2  may be —CH 2 -A 1 ; 
         R 6  is COR 11  wherein R 11  is OH; 
         R 7  is H. 
       
     
     
       2. A compound selected from the group consisting of