Organic-inorganic composite dispersion, cell culture substrate manufactured using the same, and methods for preparing the same

Abstract

Disclosed are: an organic-inorganic complex dispersion improved in film formability and adhesion to a base material. The organic-inorganic complex dispersion comprises an aqueous medium and particles of a complex dispersed in the aqueous medium, wherein the complex has a three-dimensional network structure formed by a polymer of a monomer comprising a monomer represented by general formula (1) and at least one inorganic material selected from a water-swellable clay mineral and silica. Also disclosed is an antifogging material manufactured by using the organic-inorganic complex dispersion. Further disclosed is a cell culture substratum improved in the detachability of cells cultured on the substratum, which is manufactured by using the organic-inorganic complex dispersion. Still further disclosed are manufacturing methods for same. [In the formula, R1 represents a hydrogen atom or a methyl group; R2 represents an alkylene group having 2 to 3 carbon atoms; R3 represents a hydrogen atom or an alkyl group having 1 to 2 carbon atoms; and n represents a number of 1 to 9].

The invention claimed is: 
     
       1. An organic-inorganic composite dispersion comprising particles of a composite (X) dispersed in an aqueous medium (W), the composite (X) having a three-dimensional network formed of a polymer (A) of a monomer comprising a monomer (a) represented by Formula (1) 
       
         
           
           
               
               
           
         
         where R 1  is a hydrogen atom or a methyl group, R 2  is a C 2 -C 3  alkylene group, R 3  is a C 1 -C 2  alkyl group and n is an integer of 1 to 9, 
       
       and at least one inorganic material (C) selected from a water-swellable clay mineral and silica, wherein the particles of the composite (X) have a structure in which the inorganic material (C) is uniformly dispersed in the polymer (A), the concentration (wt %) of the inorganic material (C) in the aqueous medium (W) is
 <12.4Ra+0.05 when Ra<0.19, or 
 <0.87Ra+2.17 when Ra>0.19, and 
 
       Ra is from 0.01 to 10,
 where the concentration (wt %) of the inorganic material (C) is a value calculated by dividing the weight of the inorganic material (C) by the total weight of the aqueous medium (W) and the inorganic material (C) and multiplying the resulting value by 100, and 
 Ra is a weight ratio ((C)/(A)) of the inorganic material (C) to the polymer (A). 
 
     
     
       2. An organic-inorganic composite dispersion comprising particles of a composite (X) dispersed in an aqueous medium (W), the composite (X) having a three-dimensional network formed of a polymer (A) of a monomer comprising a monomer (a) represented by Formula (1) 
       
         
           
           
               
               
           
         
         where R 1  is a hydrogen atom or a methyl group, R 2  is a C 2 -C 3  alkylene group, R 3  is a C 1 -C 2  alkyl group and n is an integer of 1 to 9, 
       
       and at least one inorganic material (C) selected from a water-swellable clay mineral and silica, wherein the particles of the composite (X) have a core-shell structure comprising a core portion in which the inorganic material (C) is dispersed in the polymer (A), and a shell portion, wherein dispersion density of the inorganic material (C) in the shell portion is higher than in the core portion, 
       wherein Ra is from 0.01 to 10, where Ra is a weight ratio ((C)/(A)) of the inorganic material (C) to the polymer (A). 
     
     
       3. A dry film of the composite (X) obtained by drying the organic-inorganic composite dispersion according to  claim 1 . 
     
     
       4. A laminate having a laminate structure comprising a support and the dry film according to  claim 3  formed on the support. 
     
     
       5. A cell culture substrate having the laminate structure according to  claim 4 . 
     
     
       6. An antifogging material having the laminate structure according to  claim 4 . 
     
     
       7. A method for preparing an organic-inorganic composite dispersion according to  claim 1 ,
 the method comprising: 
 dissolving or uniformly dispersing the monomer (a), the inorganic material (C), and a polymerization initiator (D) in the aqueous medium (W) and polymerizing the monomer (a) to form the particles of the composite (X). 
 
     
     
       8. A cell culture substrate comprising: a composite (X) obtained from the organic-inorganic composite dispersion of  claim 1 ; and
 a polymer (B) having a lower critical solution temperature, 
 wherein the content of the polymer (B) with respect to the total weight of the cell culture substrate is 0.0001% to 40% by weight. 
 
     
     
       9. The cell culture substrate according to  claim 8 , wherein the polymer (B) is exposed at a cell culture surface of the cell culture substrate. 
     
     
       10. The cell culture substrate according to  claim 8 , wherein the polymer (B) is a polymer of at least one monomer (b) selected from the group consisting of N-substituted (meth)acrylamide derivatives and N,N-di-substituted (meth)acrylamide derivatives. 
     
     
       11. The cell culture substrate according to  claim 10 , wherein the monomer (b) is at least one selected from the group consisting of N-isopropyl (meth)acrylamide, N-n-propyl (meth)acrylamide, N-cyclopropyl (meth)acrylamide, N-ethoxyethyl (meth)acrylamide, N-tetrahydrofurfuryl (meth)acrylamide, N-ethyl acrylamide, N-ethyl-N-methyl acrylamide, N,N-diethyl acrylamide, N-methyl-N-n-propyl acrylamide, N-methyl-N-isopropyl acrylamide, N-acryloyl piperidine and N-acryloyl pyrrolidine. 
     
     
       12. A method for preparing a cell culture substrate comprising: a composite (X) obtained from the organic-inorganic composite dispersion of  claim 1 ; and
 a polymer (B) having a lower critical solution temperature, 
 the method comprising: 
 a first step of mixing the monomer (a), the inorganic material (C) and a polymerization initiator (D) in an aqueous medium (W) such that the concentration of the inorganic material (C) in the aqueous medium (W) is within the range represented by the following Formula (2) or (3), and polymerizing the monomer (a) to provide a dispersion (L) of the composite (X) comprising the polymer (A) and the inorganic material (C); 
 a second step of applying the dispersion (L) to a support and drying the support to form a thin layer of the composite (X); 
 a third step of applying a solution of a water-insoluble polymerization initiator (D) in a solvent (E) to a surface (S) of the thin layer of the composite (X) and volatilizing the solvent (E); and 
 a fourth step of applying an aqueous solution of a monomer (b) undergoing polymerization to form the polymer (B) to the surface (S) and polymerizing the monomer (b) by UV irradiation. 
 
     
     
       13. A method for preparing a cell culture substrate comprising: a composite (X) obtained from the organic-inorganic composite dispersion of  claim 1 ; and
 a polymer (B) having a lower critical solution temperature, 
 the method comprising: 
 a first step of mixing the monomer (a), the inorganic material (C) and a polymerization initiator (D) in an aqueous medium (W) such that the concentration of the inorganic material (C) in the aqueous medium (W) is within the range represented by the following Formula (2) or (3), and polymerizing the monomer (a) to provide a dispersion (L) of the composite (X) comprising the polymer (A) and the inorganic material (C); and 
 a second step of adding the polymer (B) to the dispersion (L), mixing the ingredients, applying the resulting mixture to a support and drying the support. 
 
     
     
       14. A dry film of the composite (X) obtained by drying the organic-inorganic composite dispersion according to  claim 2 . 
     
     
       15. The composite dispersion according to  claim 1 , wherein the particles of the composite have a particle size of 50 nm to 5 μm. 
     
     
       16. The composite dispersion according to  claim 2 , wherein the particles of the composite have a particle size of 50 nm to 5 μm. 
     
     
       17. The composite dispersion according to  claim 2 , wherein the concentration (wt %) of the inorganic material (C) in the aqueous medium (W) is
 <12.4Ra+0.05 when Ra<0.19, or 
 <0.87Ra+2.17 when Ra≧0.19, 
 
       where the concentration (wt %) of the inorganic material (C) is a value calculated by dividing the weight of the inorganic material (C) by the total weight of the aqueous medium (W) and the inorganic material (C) and multiplying the resulting value by 100. 
     
     
       18. A laminate having a laminate structure comprising a support and the dry film according to  claim 14  formed on the support. 
     
     
       19. A cell culture substrate having the laminate structure according to  claim 18 . 
     
     
       20. An antifogging material having the laminate structure according to  claim 18 . 
     
     
       21. A method for preparing an organic-inorganic composite dispersion according to  claim 17 , the method comprising:
 dissolving or uniformly dispersing the monomer (a), the inorganic material (C), and a polymerization initiator (D) in the aqueous medium (W) and polymerizing the monomer (a) to form the particles of the composite (X). 
 
     
     
       22. A cell culture substrate comprising a composite (X) obtained from the organic-inorganic composite dispersion of  claim 2 , and a polymer (B) having a lower critical solution temperature, wherein the content of the polymer (B) with respect to the total weight of the cell culture substrate is 0.0001% to 40% by weight. 
     
     
       23. A method for preparing a cell culture substrate comprising a composite (X) obtained from the organic-inorganic composite dispersion of  claim 17 , and a polymer (B) having a lower critical solution temperature, the method comprising:
 a first step of mixing the monomer (a), the inorganic material (C) and a polymerization initiator (D) in an aqueous medium (W) such that the concentration of the inorganic material (C) in the aqueous medium (W) is within the range represented by the following Formula (2) or (3), and polymerizing the monomer (a) to provide a dispersion (L) of the composite (X) comprising the polymer (A) and the inorganic material (C); 
 a second step of applying the dispersion (L) to a support and drying the support to form a thin layer of the composite (X); 
 a third step of applying a solution of a water-insoluble polymerization initiator (D) in a solvent (E) to a surface (S) of the thin layer of the composite (X) and volatilizing the solvent (E); and 
 a fourth step of applying an aqueous solution of a monomer (b) undergoing polymerization to form the polymer (B) to the surface (S) and polymerizing the monomer (b) by UV irradiation. 
 
     
     
       24. A method for preparing a cell culture substrate comprising a composite (X) obtained from the organic-inorganic composite dispersion of  claim 17 , and a polymer (B) having a lower critical solution temperature, the method comprising:
 a first step of mixing the monomer (a), the inorganic material (C) and a polymerization initiator (D) in an aqueous medium (W) such that the concentration of the inorganic material (C) in the aqueous medium (W) is within the range represented by the following Formula (2) or (3), and polymerizing the monomer (a) to provide a dispersion (L) of the composite (X) comprising the polymer (A) and the inorganic material (C); and 
 a second step of adding the polymer (B) to the dispersion (L), mixing the ingredients, applying the resulting mixture to a support and drying the support.