Antigenic determinants of human death receptor DR5

Abstract

Provided are antigenic determinants of human death receptor DR5. The antigenic determinants have the amino acid sequence of LITQQDLAPQQRA (SEQ ID No. 7), wherein the core polypeptide is QDLAP (SEQ ID No. 1). The polypeptides comprising said antigenic determinants can activate the signal pathway downstream of DR5 after binding to monoclonal antibody AD5-10, then result in apoptosis. The antigenic determinants can be used for screening and preparing anti-human DR5 agonistic antibody, small molecular compound binding to DR5 and DR5 vaccine.

The invention claimed is: 
     
       1. A polypeptide consisting of the amino acid sequence of SEQ ID No: 1. 
     
     
       2. A nucleotide sequence consisting of the sequence of SEQ ID No: 2 encoding for the polypeptide of  claim 1 , wherein N is selected from A, T, C or G. 
     
     
       3. A method of preparing monoclonal antibodies that bind to DR5 comprising cultivating a hybridoma, which was produced by immunizing an animal with the polypeptide of  claim 1 , and recovering said antibodies.