Control of operation conditions within fluidic systems

Abstract

The invention provides methods of controlling environmental conditions within a fluidic system, where such environmental conditions can affect the operation of the system in its desired function, and fluidic channels, devices, and systems that are used in practicing these methods. Such methods are generally directed to environmental control fluids, the movement of such fluids through these systems, and the interaction of these fluids with other components of the system, e.g., other fluids or solid components of the system.

What is claimed is: 
     
       1. A method of using an environmental control reagent to maintain optimal conditions within a microfluidic device, the method comprising:
 selecting a first fluid including an environmental control reagent having a viscosity adjusting reagent; 
 introducing a first volume of the first fluid into a channel segment of a microfluidic device, the first volume of the first fluid forming a spacer fluid region completely filling a first length of the channel segment and directly contacting walls of the first length; and 
 introducing a volume of a second fluid into the channel segment of the microfluidic device adjacent to and abutting the spacer fluid region, the volume of the second fluid forming a reaction fluid region completely filling a second length of the channel segment and directly contacting walls of the second length, the second fluid comprising a component of a reaction mixture; and 
 driving the spacer fluid region and the reaction fluid region through the channel segment; 
 wherein the selecting includes selecting the viscosity adjusting reagent to provide a predetermined flow resistance to the driving, wherein viscosity of the first fluid is different than viscosity of the second fluid. 
 
     
     
       2. The method of  claim 1 , wherein the first volume of the first fluid is introduced into the channel segment after the volume of the second fluid is introduced into the channel segment. 
     
     
       3. The method of  claim 1 , wherein the volume of the second fluid is introduced into the channel segment after the first volume of the first fluid is introduced into the channel segment, the method further comprising introducing a second volume of the first fluid into the channel segment after introducing the volume of the second fluid. 
     
     
       4. The method of  claim 1 , further comprising introducing a volume of a third fluid into the channel segment. 
     
     
       5. The method of  claim 1 , wherein the second fluid comprises a first test compound. 
     
     
       6. The method of  claim 1 , wherein the second fluid comprises at least a first component of a biochemical system. 
     
     
       7. The method of  claim 1 , further comprising introducing a volume of a third fluid between the spacer fluid region and the reaction fluid region, the third fluid comprising a component of a reaction mixture. 
     
     
       8. The method of  claim 1 , wherein the viscosity adjusting reagent is selected from a group consisting of a polymeric reagent, a polysaccharide, a polysaccharide polymer, a polyacrylamide, gelatin, and a combination thereof. 
     
     
       9. The method of  claim 1  further comprising carrying out a reaction of interest in the microfluidic device, wherein the environmental control reagent is a reagent not involved directly in the reaction of interest. 
     
     
       10. The method of  claim 9 , wherein the reaction of interest is carried out within the channel segment of the microfluidic device.